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Literature summary for 2.7.11.14 extracted from
- Rhodopsin kinase: Expression in baculovirus-infected insect cells, and characterization of post-translational modifications (1997), Proc. Natl. Acad. Sci. USA, 94, 10577-10582.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| RK gene expression in baculovirus-infected Sf21 cells | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bos taurus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | enzyme is autophosphorylated | Bos taurus |
| phosphoprotein | native RK: mixture of mono- and diphosphorylated forms, two main fractions of purified recombinant RK expressed in Sf21 cells differ in their phosphorylation state: one is monophosphorylated, the other is diphosphorylated | Bos taurus |
| side-chain modification | native RK is isoprenylated, purified recombinant RK expressed in Sf21 cells: isoprenyl groups consist of mixtures of C5, C10, C15 and C20 isoprenyl moieties, no specific isoprenylation | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant RK purified from baculovirus-infected Sf21 cells: 63fold | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | retina | Bos taurus | - |
| retina | rod cell outer segment | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.097 | - |
pH 7.5, 30°C | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
| 36 | - |
about, native RK and recombinant RK expressed in Sf21 cells | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
recombinant RK expressed in Sf21 cells | Bos taurus |
| 7 | - |
native RK | Bos taurus |
| 7.5 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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