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Literature summary for 2.7.11.14 extracted from
- Structure and function in rhodopsin: Effects of disulfide cross-links in the cytoplasmic face of rhodopsin on transducin activation and phosphorylation by rhodopsin kinase (1999), Biochemistry, 38, 12893-12898.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| RK gene expression in baculovirus-infected Sf21 cells | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bos taurus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant RK purified from baculovirus-infected Sf21 cells | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | preferred substrate: light-activated form of rhodopsin, i.e. metarhodopsin II | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | effect of bovine rhodopsin mutants with disulfide cross-links between different cytoplasmic regions on the possibility to serve as substrate, only substrate is a rhodopsin mutant containing a disulfide cross-link between Cys-65 and Cys-316 | Bos taurus | ADP + phosphorhodopsin | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bos taurus |
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