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Literature summary for 2.7.11.14 extracted from
- Rhodopsin and its kinase (2001), Methods Enzymol., 343, 578-600.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| polycation | activates approximately 2fold | Bos taurus |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | mutation in RK is associated with the Oguchi disease | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| RK gene is located on chromosome 13q34 | Homo sapiens |
| RK is cloned and expressed in SF9 cells | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chelator | - |
Bos taurus | |
| Mg2+ | at high concentrations; requirement at lower concentrations | Bos taurus |  |
| polyanion | - |
Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
| Mg2+ | - |
Rattus norvegicus | |
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | 1 mM required | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | Rattus norvegicus | - |
ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | Homo sapiens | important enzyme of phototransduction | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | Bos taurus | involved in a mechanism for quenching or terminating the visual signal involving the interaction of metarhodopsin II with RK and arrestin, phosphorylation of light-activated rhodopsin by RK is the key step in the signal-termination reaction | ADP + phosphorhodopsin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | autophosphorylation of RK causes a lower affinity of enzyme for photolyzed rhodopsin allowing dissociation | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | retina | Homo sapiens | - |
| eye | retina | Bos taurus | - |
| pineal gland | - |
Rattus norvegicus | - |
| pineal gland | - |
Bos taurus | - |
| retina | - |
Homo sapiens | - |
| retina | rod cell outer segment | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | 0.7 | - |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + peptide | acid-rich peptides, RK prefers acid residues localized to the C-terminal side of the serine | Bos taurus | ADP + phosphopeptide | - |
? | |
| ATP + peptide | containing sites phosphorylated in rhodopsin | Bos taurus | ADP + phosphopeptide | - |
? | |
| ATP + rhodopsin | - |
Rattus norvegicus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | preferred substrate: ATP | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | phosphorylates bovine rhodopsin | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | phosphorylates bovine rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | highly specific for photobleached rhodopsin | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | highly specific for photobleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | RK binds to the cytoplasmic loops of photolyzed rhodopsin, forming a stable complex, and then phosphorylating it at the C-terminus, phosphorylation at different sites, including Ser-334, Ser-338 and Ser342, may play different roles in phototransduction | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | domain structure | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | important enzyme of phototransduction | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | involved in a mechanism for quenching or terminating the visual signal involving the interaction of metarhodopsin II with RK and arrestin, phosphorylation of light-activated rhodopsin by RK is the key step in the signal-termination reaction | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| GTP + rhodopsin | very poor substrate | Bos taurus | GDP + phosphorhodopsin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
| 30 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Bos taurus |
| 7.5 | - |
- |
Homo sapiens |
| 7.5 | - |
assay at, photolyzed rhodopsin substrate | Bos taurus |
| 8.5 | - |
assay at, synthetic peptide substrates | Bos taurus |
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Release 2023.1 (January 2023)
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