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Literature summary for 2.7.11.14 extracted from

  • Chen, C.K.; Hurley, J.B.
    Purification of rhodopsin kinase by recoverin affinity chromatography (2000), Methods Enzymol., 315, 404-410.
    View publication on PubMed
Search for more literature for 2.7.11.14

Cloned(Commentary)

Cloned (Comment) Organism
RK is cloned and expressed in SF9 cells Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
recoverin Ca2+-dependent inhibition Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant RK, expressed in SF9 cells, recovering affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
eye retina Bos taurus
-
retina rod cell outer segment Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
 recombinant RK expressed in SF9 cells Bos taurus

Storage Stability

Storage Stability Organism
-70°C, several months, stable Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?
ATP + rhodopsin recombinant RK expressed in SF9 cells catalyzes high-gain phosphorylation in which photoactivation of one rhodopsin molecule causes incorporation of up to several hundred phosphates into the total rhodopsin pool Bos taurus ADP + phosphorhodopsin
-
 ?
ATP + rhodopsin fully bleached rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
ATP + rhodopsin high-gain phosphorylation of rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?