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Literature summary for 2.7.11.14 extracted from
- Rhodopsin kinase: substrate specificity and factors that influence activity (1988), Biochemistry, 27, 2306-2313.
General Stability
| General Stability | Organism |
|---|---|
| stable in 6 mM dodecyl maltoside for at least 15 min | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-[p-(fluorosulfonyl)benzoyl]adenosine | pseudo-first-order kinetics, MgATP and ATP protect almost completely, rhodopsin only slightly, Mg2+ not at all | Bos taurus |  |
| Ca2+ | at concentrations equal to Mg2+, forms an unproductive Ca-ATP complex, Mg2+ partially reverses | Bos taurus | |
| EDTA | - |
Bos taurus | |
| K+ | weak, only at high concentrations | Bos taurus | |
| Mg2+ | above 10 mM, presumably by formation of Mg-ATP-Mg; requirement at lower concentrations | Bos taurus | |
| Na+ | weak, only at high concentrations | Bos taurus | |
| Peptides from cytosolic surface of rhodopsin | - |
Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters for various synthetic peptide substrates | Bos taurus | |
| 0.003 | - |
ATP | pH 7.5, 25°C | Bos taurus | |
| 0.003 | - |
rhodopsin | pH 7.5, 25°C | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | inhibitory in excess, presumably by formation of Mg-ATP-Mg | Bos taurus | |
| Mg2+ | actual substrate: Mg-ATP complex, plus activation by free Mg2+-ions | Bos taurus | |
| Mg2+ | the optimum activation is obtained with a 10:1 ratio of Mg2+/ATP | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + rhodopsin = ADP + phosphorhodopsin | mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | retina | Bos taurus | - |
| retina | rod cell outer segment | Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + peptide | containing sites phosphorylated in rhodopsin | Bos taurus | ADP + phosphopeptide | - |
? | |
| ATP + peptide | less amount of phosphoryl group incorporation than of rhodopsin | Bos taurus | ADP + phosphopeptide | - |
? | |
| ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | phosphorylates rhodopsin in the disc-membrane | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | phosphorylates bovine rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | phosphorylates rhodopsin solubilized in dodecyl maltoside | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | highly specific for photobleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | blocking of SH- and amino-groups of rhodopsin by chemical modification does not affect phosphorylation, except for succinylated rhodopsin, the binding or recognition site of enzyme contains multiple regions of rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | phosphorylates rhodopsin from cattle, rabbit, pig, alligator, best substrate: bovine rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| additional information | not: succinylated rhodopsin | Bos taurus | ? | - |
? | |
| additional information | not: glycogen synthetase | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity/pH-profile with synthetic peptide 327-347 as substrate | Bos taurus |
| 7 | - |
- |
Bos taurus |
| 7.5 | - |
assay at | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8.7 | pH 6.3: about 75% of maximal activity, pH 8.7: about half-maximal activity | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
5'-[p-(fluorosulfonyl)benzoyl]adenosine | pH 7.5, 25°C | Bos taurus | |
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