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Literature summary for 2.7.11.14 extracted from
- Rhodopsin kinase prepared from bovine rod disk membranes quenches light activation of cGMP phosphodiesterase in a reconstituted system (1986), Biochemistry, 25, 5460-5468.
General Stability
| General Stability | Organism |
|---|---|
| instability of enzyme, protease inhibitors stabilize during purification | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by Na+ | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound | Bos taurus | 16020 | - |
| membrane | rod disc membranes | Bos taurus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Bos taurus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
1 * 65000, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | enzyme is autophosphorylated in the absence of rhodopsin | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | retina | Bos taurus | - |
| eye | dark-adapted | Bos taurus | - |
| retina | rod cell outer segment | Bos taurus | - |
| retina | from dark-adapted eyes | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.062 | - |
pH 7.5, 23°C | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, highly unstable, partially purified preparation, within 4-5 days, 90% loss of activity, mixture of protease inhibitors stabilizes for several weeks | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | phosphorylates bovine rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| additional information | enzyme quenches light activation of cGMP phosphodiesterase in a reconstituted system | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 65000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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