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Literature summary for 2.7.11.14 extracted from
- Mechanism of rhodopsin kinase activation (1991), J. Biol. Chem., 266, 12949-12955.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| mastoparan | stimulates, mechanism | Bos taurus |  |
| additional information | various enzymatically truncated forms of photolyzed rhodopsin stimulate, light-dependent | Bos taurus | |
| rhodopsin | activation, allosteric, the kinase binds to cytoplasmic loops of rhodopsin | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km values for truncated forms of rhodopsin | Bos taurus | |
| 0.004 | 0.0044 | rhodopsin | pH 7.5, 30°C | Bos taurus | |
| 7.1 | - |
DDEASTTVSKTETSQVARRR | pH 7.5, 30°C, peptide C | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | rod membranes | Bos taurus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + rhodopsin = ADP + phosphorhodopsin | mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | retina | Bos taurus | - |
| retina | rod cell outer segment | Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + DDEASTTVSKTETSQVARRR | synthetic peptide C, very poor substrate | Bos taurus | ? | - |
? | |
| ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | only phosphorylates C-terminal sites of rhodopsin, role of the cytoplasmic loops and C-terminal region of rhodopsin in binding and activating enzyme, V-VI loop is crucial for kinase binding, truncated forms of rhodopsin as substrates | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | phosphorylates bovine rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bos taurus |
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