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Literature summary for 2.7.11.14 extracted from

  • Palczewski, K.; Buczylko, J.; Van Hooser, P.; Carr, S.A.; Huddleston, M.J.; Crabb, J.W.
    Identification of the autophosphorylation sites in rhodopsin kinase (1992), J. Biol. Chem., 267, 18991-18998.
    View publication on PubMed
Search for more literature for 2.7.11.14

Activating Compound

Activating Compound Comment Organism Structure
rhodopsin activates Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by a synthetic peptide corresponding to the major or minor autophosphorylation site of RK Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane rod membranes Bos taurus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, dephosphorylated RK, SDS-PAGE Bos taurus
66000
-
 x * 66000, autophosphorylated RK, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + rhodopsin Bos taurus second messenger-independent protein kinase, involved in the deactivation of photolyzed rhodopsin ADP + phosphorhodopsin
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Bos taurus P28327
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information putative posttranslational modification sites: C-terminal isoprenylation, N-terminal myristoylation Bos taurus
phosphoprotein autophosphorylation sites of RK, major sites are Ser-488 and Thr-489, minor site is Ser-21 Bos taurus
phosphoprotein autophosphorylation may lower the affinity of RK for photoactivated rhodopsin via repulsion between phosphorylated sites on photoactivated rhodopsin and RK Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
eye retina Bos taurus
-
retina rod cell outer segment Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.555
-
 pH 7.5 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + peptide low catalytic efficiency of RK toward a peptide containing its major autophosphorylation site Bos taurus ADP + phosphopeptide
-
 ?
ATP + protein autophosphorylation Bos taurus ADP + phosphoprotein
-
 ?
ATP + rhodopsin highly specific for photobleached rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
ATP + rhodopsin high-gain phosphorylation of rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
ATP + rhodopsin domain structure Bos taurus ADP + phosphorhodopsin
-
 ?
ATP + rhodopsin second messenger-independent protein kinase, involved in the deactivation of photolyzed rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, autophosphorylated RK, SDS-PAGE Bos taurus
? x * 65000, dephosphorylated RK, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
rhodopsin kinase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus
30
-
 peptide assay Bos taurus