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Literature summary for 2.7.11.13 extracted from

  • Abeyweera, T.P.; Rotenberg, S.A.
    Design and characterization of a traceable protein kinase Calpha (2007), Biochemistry, 46, 2364-2370.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of FLAG-tagged wild-type and mutant isozyme PLCalpha in MCF-10A human breast epithelial cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
M417A site-directed mutagenesis, utilizes the alternate cofactor N6-phenyl-ATP Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0093
-
ATP pH 7.4, 37°C, recombinant wild-type isozyme PKCalpha Homo sapiens
0.0124
-
N6-phenyl-ATP pH 7.4, 37°C, recombinant isozyme PKCalpha mutant M417A Homo sapiens
0.0828
-
ATP pH 7.4, 37°C, recombinant isozyme PKCalpha mutant M417A Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens
Mg2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isozyme pKCalpha
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + EF factor 1 i.e. eukaryotic translation elongation factor 1-alpha1, in vitro phosphorylation Homo sapiens ADP + phosphorylated EF factor 1
-
?
ATP + histone H2 in vitro phosphorylation Homo sapiens ADP + phosphorylated histone H2
-
?
ATP + histone H3 in vitro phosphorylation Homo sapiens ADP + phosphorylated histone H3
-
?
ATP + lamin A a nuclear membrane protein, isozyme PKCalpha Homo sapiens ADP + phosphorylated lamin A
-
?
ATP + lamin C a nuclear membrane protein, isozyme PKCalpha, phosphorylation at Ser572 Homo sapiens ADP + phosphorylated lamin C
-
?
ATP + RFARKGSLRQKNV synthetic peptide substrate Homo sapiens ADP + RFARKGphosphoSLRQKNV
-
?
N6-phenyl-ATP + RFARKGSLRQKNV synthetic peptide substrate, recombinant isozyme PKCalpha mutant M417A Homo sapiens N6-phenyl-ADP + RFARKGphosphoSLRQKNV
-
?

Synonyms

Synonyms Comment Organism
PKC
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP preferred by the wild-type PKCalpha Homo sapiens
N6-phenyl-ATP preferred by PKCalpha mutant M417A to phosphorylate peptide and protein substrates, no activity with the wild-type PKCalpha Homo sapiens