Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged wild-type and mutant isozyme PLCalpha in MCF-10A human breast epithelial cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
M417A | site-directed mutagenesis, utilizes the alternate cofactor N6-phenyl-ATP | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0093 | - |
ATP | pH 7.4, 37°C, recombinant wild-type isozyme PKCalpha | Homo sapiens | |
0.0124 | - |
N6-phenyl-ATP | pH 7.4, 37°C, recombinant isozyme PKCalpha mutant M417A | Homo sapiens | |
0.0828 | - |
ATP | pH 7.4, 37°C, recombinant isozyme PKCalpha mutant M417A | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme pKCalpha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + EF factor 1 | i.e. eukaryotic translation elongation factor 1-alpha1, in vitro phosphorylation | Homo sapiens | ADP + phosphorylated EF factor 1 | - |
? | |
ATP + histone H2 | in vitro phosphorylation | Homo sapiens | ADP + phosphorylated histone H2 | - |
? | |
ATP + histone H3 | in vitro phosphorylation | Homo sapiens | ADP + phosphorylated histone H3 | - |
? | |
ATP + lamin A | a nuclear membrane protein, isozyme PKCalpha | Homo sapiens | ADP + phosphorylated lamin A | - |
? | |
ATP + lamin C | a nuclear membrane protein, isozyme PKCalpha, phosphorylation at Ser572 | Homo sapiens | ADP + phosphorylated lamin C | - |
? | |
ATP + RFARKGSLRQKNV | synthetic peptide substrate | Homo sapiens | ADP + RFARKGphosphoSLRQKNV | - |
? | |
N6-phenyl-ATP + RFARKGSLRQKNV | synthetic peptide substrate, recombinant isozyme PKCalpha mutant M417A | Homo sapiens | N6-phenyl-ADP + RFARKGphosphoSLRQKNV | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKC | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | preferred by the wild-type PKCalpha | Homo sapiens | |
N6-phenyl-ATP | preferred by PKCalpha mutant M417A to phosphorylate peptide and protein substrates, no activity with the wild-type PKCalpha | Homo sapiens |