Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | PKC activation is influenced by intracellular Ca2+ concentration and activity of the metabotropic glutamate receptor 5 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged wild-type PKC isozymes in HEK-293 cells, and of GFP-tagged PKCgamma wild-type and mutants lacking the C1 or C2 domain in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | downregulation of PKCdelta by short interfering RNA, deletion of domain C2 of PKCgamma leads to complete loss of activity, while deletion of C1 domain shows no effect on PKCgamma activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-methyl-6-(phenylethynyl)-pyridine | complete inhibition of PKCgamma oscillation at 0.05 mM | Homo sapiens | |
arachidonoyl fluoromethylketone | inhibition of PKCgamma oscillation at 0.1 mM | Homo sapiens | |
bromoerol lactone | slight inhibition of PKCgamma oscillation at 0.1 mM | Homo sapiens | |
compound 48/80 | complete inhibition of PKCgamma oscillation at 0.1 mM | Homo sapiens | |
cytochalasine D | inhibition of PKCgamma oscillation at 0.01 mM | Homo sapiens | |
ethanol | 0.03%, inhibition of PKCgamma oscillation at 0.1 mM | Homo sapiens | |
additional information | no inhibition of PKCgamma oscillation by EGTA, aristolochic acid, and colchicine | Homo sapiens | |
thapsigargin | complete inhibition of PKCgamma oscillation at 0.005 mM | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | translocation of PKCgamma from cytosol to membrane | Homo sapiens | 5829 | - |
plasma membrane | translocation of PKCgamma from cytosol to membrane, PKCgamma oscillates on the membrane induced by glutamate and activated metabotropic glutamate receptor 5, overview | Homo sapiens | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on, depends on the isozyme, e.g. isozyme betaII and gamma are dependent on Ca2+, while isozyme delta is not | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + metabotropic glutamate receptor 5 | Homo sapiens | isozyme-specific phosphorylation of metabotropic glutamate receptor 5 by PKCdelta blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKCgamma | ADP + phosphorylated metabotropic glutamate receptor 5 | - |
? | |
additional information | Homo sapiens | activation of metabotropic glutamate receptor 5 induces translocation of PKCgamma and PKCdelta to plasma membrane and elicits cyclical translocations of myristoylated alanine-rich PKC substrate from plasma membrane to cytosol | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
several isozymes | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + metabotropic glutamate receptor 5 | isozyme-specific phosphorylation of metabotropic glutamate receptor 5 by PKCdelta blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKCgamma | Homo sapiens | ADP + phosphorylated metabotropic glutamate receptor 5 | - |
? | |
ATP + metabotropic glutamate receptor 5 | phosphorylation at Thr840, PKCdelta is active with wild-type substrate and mutant T840A, but not with mutant T840D, no phosphorylation by PKCgamma | Homo sapiens | ADP + phosphorylated metabotropic glutamate receptor 5 | - |
? | |
additional information | activation of metabotropic glutamate receptor 5 induces translocation of PKCgamma and PKCdelta to plasma membrane and elicits cyclical translocations of myristoylated alanine-rich PKC substrate from plasma membrane to cytosol | Homo sapiens | ? | - |
? | |
additional information | myristoylated alanine-rich PKC substrate is repetitively phosphorylated by oscillating gammaPKC on the plasma membrane | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKC | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |