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Literature summary for 2.7.11.12 extracted from

  • El Bakkouri, M.; Kouidmi, I.; Wernimont, A.K.; Amani, M.; Hutchinson, A.; Loppnau, P.; Kim, J.J.; Flueck, C.; Walker, J.R.; Seitova, A.; Senisterra, G.; Kakihara, Y.; Kim, C.; Blackman, M.J.; Calmettes, C.; Baker, D.A.; Hui, R.
    Structures of the cGMP-dependent protein kinase in malaria parasites reveal a unique structural relay mechanism for activation (2019), Proc. Natl. Acad. Sci. USA, 116, 14164-14173 .
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
cGMP
-
 Plasmodium vivax
cGMP
-
 Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Plasmodium vivax
expressed in Escherichia coli BL21(DE3) cells Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, apoenzyme using 10% (w/v) PEG 5000 monomethyl ether, 5% (w/v) tascimate, 0.1 M HEPES, 15 mM spermidine (pH 7.0), and 25% (v/v) glycerol, enzyme bound to AMPPNP using 18.2% (w/v) PEG 3350, 0.1 M HEPES (pH 7.0) Plasmodium vivax
sitting drop vapor diffusion method, using 0.4 M L-proline, 10% poly(ethylene glycol) 3350, 0.1 M HEPES (pH 7.8), and 15% (w/v) ethylene glycol Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
E589A inactive Plasmodium vivax
E589A inactive Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8I719
-
-
Plasmodium vivax A5K0N4
-
-
Plasmodium vivax Salvador A5K0N4
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel bead chromatography and Superdex 200 gel filtration Plasmodium vivax
nickel bead chromatography and Superdex 200 gel filtration Plasmodium falciparum

Subunits

Subunits Comment Organism
dimer x-ray crystallography Plasmodium falciparum
homodimer x-ray crystallography Plasmodium vivax

Synonyms

Synonyms Comment Organism
cyclic guanosine-3',5'-monophosphate-dependent protein kinase
-
 Plasmodium vivax
cyclic guanosine-3',5'-monophosphate-dependent protein kinase
-
 Plasmodium falciparum
PKG
-
 Plasmodium vivax
PKG
-
 Plasmodium falciparum
protein kinase G
-
 Plasmodium vivax
protein kinase G
-
 Plasmodium falciparum