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Literature summary for 2.7.11.12 extracted from

  • Collins, C.R.; Hackett, F.; Strath, M.; Penzo, M.; Withers-Martinez, C.; Baker, D.A.; Blackman, M.J.
    Malaria parasite cGMP-dependent protein kinase regulates blood stage merozoite secretory organelle discharge and egress (2013), PLoS Pathog., 9, e1003344.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
cyclic nucleotide-binding domain CNB-D in the apo and cGMP bound states, hanging drop vapor diffusion method, using 0.2 M lithium sulfate, 15% ethanol, 0.1 M citrate at pH 5.5 and 10% (w/v) 1,5-diaminopentane dihydrochloride Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
D533A the mutant shows strongly reduced activity compared to the wild type enzyme Plasmodium falciparum
Q532A the mutant shows strongly reduced activity compared to the wild type enzyme Plasmodium falciparum
R484A the mutant shows strongly reduced activity compared to the wild type enzyme Plasmodium falciparum
R484A/Q532A/D533A the mutant shows strongly reduced activity compared to the wild type enzyme Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a protein Plasmodium falciparum
-
 ADP + phosphorylated protein
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
IMAC nickel column chromatography and Superdex 75 gel filtration Plasmodium falciparum

Source Tissue

Source Tissue Comment Organism Textmining
merozoite
-
 Plasmodium falciparum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a protein
-
 Plasmodium falciparum ADP + phosphorylated protein
-
 ?

Synonyms

Synonyms Comment Organism
PKG
-
 Plasmodium falciparum

General Information

General Information Comment Organism
malfunction blocking of the enzyme stops both replication and transmission of the Plasmodium falciparum Plasmodium falciparum