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Literature summary for 2.7.11.12 extracted from

  • Kyle, B.D.; Hurst, S.; Swayze, R.D.; Sheng, J.; Braun, A.P.
    Specific phosphorylation sites underlie the stimulation of a large conductance, Ca2+-activated K+ channel by cGMP-dependent protein kinase (2013), FASEB J., 27, 2027-2038.
    View publication on PubMed
Search for more literature for 2.7.11.12

Activating Compound

Activating Compound Comment Organism Structure
cGMP activates cGKI Rattus norvegicus
N2,2'-O-dibutyryl-cGMP a membrane-permeable cGMP analogue, activates cGKI Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
transient co-expression of cGKI and substrate BKCa channels in HEK 293 cells Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Bos taurus
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-
-
Rattus norvegicus
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
aorta
-
 Rattus norvegicus
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smooth muscle cell aortic A7r5 smooth muscle cells Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + BKCa channel protein phosphorylation at mutiple serine residues, recombinant expressed Mus musculus BKCa channels Bos taurus ADP + BKCa channel phosphoprotein
-
 ?
ATP + BKCa channel protein phosphorylation at Ser691, Ser873, and Ser1112 on recombinant Mus musculus BKCa channels expressed in Rattus norvegicus aortic A7r5 smooth muscle cells or in humn in HEK 293 cells Rattus norvegicus ADP + BKCa channel phosphoprotein
-
 ?
additional information serine to alanine substitutions at 3 of 6 putative cGKI phosphorylation sites, Ser691, Ser873, and Ser1112, in the BKCa alpha-subunit and mutant expression in A7r5 cells individually reduce direct channel phosphorylation by 25-60% and block BKCa activation by either an NO donor or a membrane-permeable cGMP by 80-100% Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
cGKI
-
 Rattus norvegicus
cGKIalpha
-
 Bos taurus
type I cGMP-dependent protein kinase
-
 Rattus norvegicus
type Ialpha cGMP-dependent protein kinase
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus
ATP
-
 Bos taurus

General Information

General Information Comment Organism
malfunction acute inhibition of cGKI prevented stimulus-evoked enhancement of BKCa channel activity Bos taurus
malfunction acute inhibition of cGKI prevents stimulus-evoked enhancement of BKCa channel activity Rattus norvegicus
physiological function the activity of large conductance, Ca2+-activated K+ channels, i.e. BKCa channels, can be increased after modulation by type I cGMP-dependent protein kinase via nitric oxide/cGMP signaling Rattus norvegicus
physiological function the activity of large conductance, Ca2+-activated K+ channels, i.e. BKCa channels, can be increased after modulation by type I cGMP-dependent protein kinase via nitric oxide/cGMP signaling Bos taurus