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Literature summary for 2.7.11.12 extracted from

  • Richie-Jannetta, R.; Busch, J.L.; Higgins, K.A.; Corbin, J.D.; Francis, S.H.
    Isolated regulatory domains of cGMP-dependent protein kinase Ialpha and Ibeta retain dimerization and native cGMP-binding properties, and undergo isoform-specific conformational changes (2006), J. Biol. Chem., 281, 6977-6984.
    View publication on PubMed
Search for more literature for 2.7.11.12

Activating Compound

Activating Compound Comment Organism Structure
cGMP two allosteric binding sites in the regulatory domains in PKGI isozymes, structure and mechanism, enzyme elongation by cGMP binding ehich induces conformational changes, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of PKGIalpha DELTA349-670 and PKGIbeta DELTA364-685 consisting of the regulatory domains in Spodoptera frugiperda Sf9 cells using the baculovirus infection system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information PKGI contains autoinhibitory subdomains in the regulatory domains and two allosteric cGMP-binding sites of isozymes alpha and beta Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information cGMP binding kinetics for recombinant regulatory domains of PKGI isozymes Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, about, recombinant PKGIalpha DELTA349-670, sequence calculation, 2 * 41000, about, recombinant PKGIbeta DELTA364-685, sequence calculation Homo sapiens
41000
-
 2 * 40000, about, recombinant PKGIalpha DELTA349-670, sequence calculation, 2 * 41000, about, recombinant PKGIbeta DELTA364-685, sequence calculation Homo sapiens
91000
-
 recombinant PKGIalpha DELTA349-670, sucrose density gradient centrifugation Homo sapiens
99000
-
 recombinant PKGIbeta DELTA364-685, sucrose density gradient centrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 two isozymes of PKGI, PKGIalpha and Ibeta
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PKGIalpha DELTA349-670 and PKGIbeta DELTA364-685 from Sf9 insect cells by 8-aminohexylamino-cAMP affinity chromatography and gel filtration Homo sapiens

Subunits

Subunits Comment Organism
dimer 2 * 40000, about, recombinant PKGIalpha DELTA349-670, sequence calculation, 2 * 41000, about, recombinant PKGIbeta DELTA364-685, sequence calculation Homo sapiens
More dimerization of isozymes PKGIalpha and Ibeta, each subunit contains a regulatory R domain and a catalytic C domain Homo sapiens

Synonyms

Synonyms Comment Organism
cGMP-dependent protein kinase I
-
 Homo sapiens
PKGI
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens