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Literature summary for 2.7.11.12 extracted from
- Dimerization of cGMP-dependent protein kinase Ibeta is mediated by an extensive amino-terminal leucine zipper motif, and dimerization modulates enzyme function (2003), J. Biol. Chem., 278, 50070-50079.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cGMP | - |
Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| placental PKG Ibeta, expression of wild-type and mutant enzymes in Escherichia coli and in Spodoptera frugiperda Sf9 cells using the baculovirus infection system | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I24A | site-directed mutagenesis, unaltered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
| L17A | site-directed mutagenesis, unaltered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
| L17A/I24A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
| L17A/I24A/L31A/I38A/L45A/I52A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
| L31A/I38A | site-directed mutagenesis, unaltered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
| L31A/I38A/L45A/I52A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
| L3A/L10A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
| L3A/L10A/L17A/I24A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
| L3A/L10A/L45A/I52A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
| additional information | construction of a PKG Ibeta leucine zipper mutant, and of the deletion mutant DELTA1-52 lacking the N-terminal part, mutants show slightly altered properties, overview | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics and dissociation constants of cGMP | Homo sapiens | |
| 0.0104 | - |
ATP | pH 7.4, 30°C, recombinant PKG Ibeta deletion mutant DELTA1-52 | Homo sapiens | |
| 0.0133 | - |
ATP | pH 7.4, 30°C, recombinant wild-type PKG Ibeta | Homo sapiens | |
| 0.0139 | - |
ATP | pH 7.4, 30°C, recombinant PKG Ibeta leucine zipper mutant | Homo sapiens | |
| 0.0263 | - |
RKRSRAE | pH 7.4, 30°C, recombinant PKG Ibeta deletion mutant DELTA1-52 | Homo sapiens | |
| 0.0304 | - |
RKRSRAE | pH 7.4, 30°C, recombinant wild-type PKG Ibeta | Homo sapiens | |
| 0.0313 | - |
RKRSRAE | pH 7.4, 30°C, recombinant PKG Ibeta leucine zipper mutant | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
recombinant deletion mutant DELTA1-52, monomeric form, sedimentation equilibrium analysis | Homo sapiens |
| 86000 | - |
recombinant PKG Ibeta mutant L17A/I24A, monomeric form at 0.95 nM concentration, sedimentation equilibrium analysis | Homo sapiens |
| 88000 | - |
recombinant PKG Ibeta mutant L31A/I38A, monomeric form at 0.95 nM concentration, sedimentation equilibrium analysis | Homo sapiens |
| 89000 | - |
recombinant PKG Ibeta mutant L3A/L10A/L45A/I52A, monomeric form, sedimentation equilibrium analysis | Homo sapiens |
| 92000 | - |
recombinant PKG Ibeta mutant L31A/I38A/L45A/I52A, monomeric form, sedimentation equilibrium analysis | Homo sapiens |
| 96000 | - |
recombinant PKG Ibeta mutant L3A/L10A/L17A/I24A, monomeric form, sedimentation equilibrium analysis | Homo sapiens |
| 98000 | - |
recombinant PKG Ibeta leucine zipper mutant, monomeric form, sedimentation equilibrium analysis | Homo sapiens |
| 120000 | - |
recombinant PKG Ibeta mutant L17A/I24A, dimeric form at 51 nM concentration, sedimentation equilibrium analysis | Homo sapiens |
| 140000 | - |
recombinant PKG Ibeta mutant L31A/I38A, dimeric form at 51 nM concentration, sedimentation equilibrium analysis | Homo sapiens |
| 170000 | - |
recombinant PKG Ibeta mutants L3A/L10A, L17A, and I24A, dimeric forms, sedimentation equilibrium analysis | Homo sapiens |
| 180000 | - |
recombinant wild-type PKG Ibeta, dimeric form, sedimentation equilibrium analysis | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
females | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant PKG Ibeta from Sf9 insect cells by 8-amino-hexylamino-cAMP affinity chromatography and gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + RKRSRAE | commercial synthetic peptide substrate | Homo sapiens | ADP + RKRS(P)RAE | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | dimerization of PKG Ibeta via the N-terminal dimerization domain containing an extensive leucine zipper motif modifying enzyme activity | Homo sapiens |
| More | analysis of the leucine zipper motif within the N-terminal dimerization domain, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cGKIbeta | - |
Homo sapiens |
| cGMP-dependent protein kinase Ibeta | - |
Homo sapiens |
| PKG Ibeta | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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