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Literature summary for 2.7.11.12 extracted from
- Expression of constitutively active cGMP-dependent protein kinase prevents glucose stimulation of thrombospondin 1 expression and TGF-beta activity (2003), Diabetes, 52, 2144-2150.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cGMP | required by the full length enzyme for activity, the activity of the recombinant catalytic domain is independent of cGMP | Homo sapiens |  |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | gene transfer of the catalytic domain of the enzyme might provide a new strategy for treatment of diabetic renal fibrosis | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| stable tetracycline-inducible expression of the catalytic enzyme domain in Rattus norvegicus mesangial cells leading to suppression of glucose-induced expression of thrombospondin 1 and thus of transforming growth factor-beta activaation and the TGF-beta-dependent expression of fibronectin and collagene type IV | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of constitutively active enzyme prevents glucose stimulation of thrombospondin 1 expression and TGF-beta activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | high levels reduce enzyme activity in vivo leading to increased thrombospondin 1-dependent activation of transforming growth factor-beta, and increased TGF-beta-dependent expression of fibronectin and collagene type IV | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the enzyme negatively regulates gene expression of thrombospondin 1, which activates the transforming growth factor-beta, at both transcriptional and posttranscriptional level inhibiting the fibrogenic potential fo high D-glucose levels, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.005 | - |
recombinant catalytic domain in rat mesangial cell extract in absence of cGMP | Homo sapiens |
| 0.011 | - |
recombinant catalytic domain in rat mesangial cell extract in presence of cGMP | Homo sapiens |
| 0.02 | - |
recombinant tetracyclin-induced catalytic domain in rat mesangial cell extract in absence of cGMP | Homo sapiens |
| 0.026 | - |
recombinant tetracyclin-induced catalytic domain in rat mesangial cell extract in presence of cGMP | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme negatively regulates gene expression of thrombospondin 1, which activates the transforming growth factor-beta, at both transcriptional and posttranscriptional level inhibiting the fibrogenic potential fo high D-glucose levels, overview | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PKG | - |
Homo sapiens |
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