Literature summary for 2.7.11.12 extracted from

Xue, J.; Milburn, P.J.; Hanna, B.T.; Graham, M.E.; Rostas, J.A.; Robinson, P.J.
Phosphorylation of septin 3 on Ser-91 by cGMP-dependent protein kinase-I in nerve terminals (2004), Biochem. J., 381, 753-760.

Search for more literature for 2.7.11.12

Activating Compound

Activating Compound	Comment	Organism	Structure
8-pCPT-CGMP	i.e. 8-(4-chlorophenylthio)guanosine 3',5'-cyclic monophosphate	Bos taurus
cGMP	-	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0865	-	septin 3 peptide 86-98	pH 7.4, 30°C, PKG-I	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
lung	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + septin 3	substrate from rat nerves: recombinant His-tagged septin 3 expressed in Escherichia coli or purified from brain and prepared in synaptosomes, phosphorylation at Ser91 and Ser92, no activity with septin 3 mutants S91A and S92A	Bos taurus	ADP + phosphorylated septin 3	-	?
ATP + septin 3 peptide 86-98	-	Bos taurus	ADP + phosphorylated septin 3 peptide 86-98	-	?

Synonyms

Synonyms	Comment	Organism
cGMP-dependent protein kinase-I	-	Bos taurus
PKG-I	-	Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Bos taurus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Bos taurus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)