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Literature summary for 2.7.11.11 extracted from

  • Schauble, S.; King, C.C.; Darshi, M.; Koller, A.; Shah, K.; Taylor, S.S.
    Identification of ChChd3 as a novel substrate of the cAMP-dependent protein kinase (PKA) using an analog-sensitive catalytic subunit (2007), J. Biol. Chem., 282, 14952-14959.
    View publication on PubMed
Search for more literature for 2.7.11.11

Activating Compound

Activating Compound Comment Organism Structure
cAMP
-
 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of His-tagged mutant catalytic subunit M120G in Escherichia coli strain BL21(DE3) requiring co-expression with PDK1 for stabilization, overview Mus musculus

Protein Variants

Protein Variants Comment Organism
M120G site-directed mutagenesis in the ATP-binding pocket of the catalytic subunit, engineering of the PKA catalytic subunit to accept bulky N6-substituted ATP analogs, using a chemical genetics approach initially pioneered with v-Src, overview, N6(benzyl)-ATP and N6(phenethyl)-ATP are the prefrred cofactors of the mutant Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
N6-benzyl-ATP preferred co-substrate of catalytic subunit mutant M120G, but inhibitory for the mutant, not the wild-type enzyme, versus kemptide, overview Mus musculus
N6-phenethyl-ATP preferred co-substrate of catalytic subunit mutant M120G, but inhibitory for the mutant, not the wild-type enzyme, versus kemptide, overview Mus musculus
regulatory subunit 1alpha
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 N6-benzyl-ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
0.0015
-
 N6-phenethyl-ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
0.0174
-
 ATP pH 7.0, 37°C, recombinant wild-type catalytic subunit Mus musculus
0.0305
-
 ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
0.1
-
 N6-benzyl-ATP above, pH 7.0, 37°C, recombinant wild-type catalytic subunit Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Mus musculus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the catalytically active subunit mutant M120G is phosphorylated on Thr197 and Ser338 Mus musculus

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged mutant catalytic subunit M120G from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ChChd3 an endogenous mitochondrial protein Mus musculus ADP + phosphorylated ChChd3
-
 ?
ATP + kemptide
-
 Mus musculus ADP + phospho-kemptide
-
 ?
N6-benzyl-ATP + ChChd3 cofactor of mutant M120G, poor activity with the wild-type catalytic subunit Mus musculus N6-benzyl-ADP + phosphorylated ChChd3
-
 ?
N6-phenethyl-ATP + ChChd3 cofactor of mutant M120G, poor activity with the wild-type catalytic subunit Mus musculus N6-phenethyl-ADP + phosphorylated ChChd3
-
 ?

Synonyms

Synonyms Comment Organism
PKA
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 N6-phenethyl-ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
0.5
-
 N6-benzyl-ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
10.6
-
 ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
19.6
-
 ATP pH 7.0, 37°C, recombinant wild-type catalytic subunit Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP preferred cofactor of the wild-type catalytic subunit Mus musculus
N6-benzyl-ATP preferred co-substrate of catalytic subunit mutant M120G, but inhibitory versus kemptide, overview Mus musculus
N6-phenethyl-ATP preferred co-substrate of catalytic subunit mutant M120G, but inhibitory versus kemptide, overview Mus musculus