Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | dependent on | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of catalytic subunit PKA Calpha in Escherichia coli strain BL21 | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant catalytic apo-subunit apo-PKA Calpha phosphorylated at Ser10, Thr197, and Ser338, hanging drop vapour diffusion method, 4°C, 0.02 ml of 7.5 mg/ml protein in solution 0.1 mM bicine buffer, pH 8.0, 150 mM ammonium acetate, 10 mM 2-mercaptoethanol, and 3% 2-methyl-2,4-pentanediol versus 1 ml reservoir solution containing 0.1 mM Tris-HCl, pH 7.5, and 10-20% v/v 2-methyl-2,4-pentanediol, temperature-sensitive crystals need 6 months to 1 year to grow, cryoprotection by a gradient of 2-methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
IP20 | iodinated inhibitor peptide substrate | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | as MgATP2-, binding site structure | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40800 | - |
x * 40800, recombinant catalytic subunit PKA Calpha, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme autophosphorylates at Ser10, Thr197, and Ser338 of catalytic subunit PKA Calpha | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant catalytic subunit PKA Calpha from Escherichia coli strain BL21 by P11 cellulose and ion exchange chromatography | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | active site residues and structure, mechanism of ligand binding and ligand-induced conformational changes | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + SP20 | substrate peptide, the C subunit SP20-binding residues are E203, F129, E170, E230, D166, and K168 | Mus musculus | ADP + SP20 phosphate | - |
? | |
additional information | the enzyme autophosphorylates at Ser10, Thr197, and Ser338 of PKA Calpha, substrate-induced conformational changes | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40800, recombinant catalytic subunit PKA Calpha, SDS-PAGE | Mus musculus |
More | apoenzyme structure analysis, hydrophobic core network, overview | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
PKA Calpha | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | as MgATP2-, binding site structure | Mus musculus |