Literature summary for 2.7.11.11 extracted from

Iyer, G.H.; Moore, M.J.; Taylor, S.S.
Consequences of lysine 72 mutation on the phosphorylation and activation state of cAMP-dependent kinase (2005), J. Biol. Chem., 280, 8800-8807.

Search for more literature for 2.7.11.11

Activating Compound

Activating Compound	Comment	Organism	Structure
cAMP	dependent on	Mus musculus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of PKA catalytic subunit Calpha as His-tagged protein in Escherichia coli strain BL21(DE3) and of HA-tagged wild-type and mutant PKA Calpha in COS cells	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
K72A	site-directed mutagenesis, inactive unphosphorylated mutant	Mus musculus
K72H	site-directed mutagenesis, inactive unphosphorylated mutant	Mus musculus
K72M	site-directed mutagenesis, inactive unphosphorylated mutant	Mus musculus
K72R	site-directed mutagenesis, inactive unphosphorylated mutant	Mus musculus
S338A	site-directed mutagenesis, mutant is phosphorylated at T197, reduced activity compared to the wild-type enzyme	Mus musculus
T197A	site-directed mutagenesis, mutant is not phosphorylated, inactive mutant	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
H-89	inhibits autophosphorylation of the catalytic subunit C, reversible	Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation at T197 by exogenous kinase PDK-I, the enzyme performs autophosphorylation at K72, S338 and T197, the latter is phosphorylated first and required for activity, phosphorylation of the other 2 positions take place only if T197 is already phosphorylated, mechanism	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + Kemptide	LRRASLG peptides substrate	Mus musculus	ADP + phosphorylated Kemptide	LRRA-phosphoserine-LG	?
additional information	the enzyme performs autophosphorylation at K72, S338, and T197	Mus musculus	?	-	?

Synonyms

Synonyms	Comment	Organism
PKA	-	Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mus musculus

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Release 2023.1 (January 2023)