Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | dependent on, acts synergistically with cGMP, binding site domain structure on the regulatory subunit RII | Apis mellifera | |
cGMP | activates, acts synergistically with cAMP | Apis mellifera | |
additional information | phosphate-binding cassettes PCB of domain A and B of the regulatory subunits | Apis mellifera |
Cloned (Comment) | Organism |
---|---|
regulatory subunit RII, DNA sequence determination and analysis, phylogenetic analysis | Apis mellifera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Apis mellifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis mellifera | Q6ZXJ1 | - |
- |
Purification (Comment) | Organism |
---|---|
PKAII holoenzyme from bee brain by ion exchange, adsorption, and cAMP-affinity chromatography, thereby dissociation of regulatory and catalytic subunits, and reconstitution | Apis mellifera |
Renatured (Comment) | Organism |
---|---|
reconstitution of holoenzyme from purified subunits in 50 mM Tris-HCl, pH 7.8, 1 mM EDTA, 1 mM EGTA, 10 mM 2-mercaptoethanol, and 0.1 M NaCl | Apis mellifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Apis mellifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phosphatase inhibitor PKI | - |
Apis mellifera | ADP + phosphorylated phosphatase inhibitor PKI | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure, deduced from DNA and amino acid sequence, analysis and comparison | Apis mellifera |
Synonyms | Comment | Organism |
---|---|---|
PKAII | - |
Apis mellifera |
protein kinase A type II | - |
Apis mellifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Apis mellifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Apis mellifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Apis mellifera |