Literature summary for 2.7.11.10 extracted from

Kim, Y.L.; Kim, J.E.; Shin, K.J.; Lee, S.; Ahn, C.; Chung, J.; Kim, D.H.; Seong, J.Y.; Hwang, J.I.
GbetaL regulates TNFalpha-induced NF-kappaB signaling by directly inhibiting the activation of IkappaB kinase (2008), Cell. Signal., 20, 2127-2133.

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Cloned(Commentary)

Cloned (Comment)	Organism
IKKbeta DNA fragments encoding the region covering the RelB-transactivation domain containing a putative leucine zipper motif, aa301-540, or helix-loop-helix and NEMO-binding domain, aa541-757, are inserted into pBHA and used as baites to screen a cDNA library, to express GST-fusion proteins in Escherichia coli, the gene fragments aa1-300, aa301-540, and aa541-756 are inserted into pGEX4T-1 or pCDNA3HA, all IKKbeta constructs, full length, aa1-300, aa301-757, aa1-540, and aa301-540-deleted forms, and IKKalpha are inserted into pCMV2-FLAG plasmids	Mus musculus

Cloned (Comment)

Organism

IKKbeta DNA fragments encoding the region covering the RelB-transactivation domain containing a putative leucine zipper motif, aa301-540, or helix-loop-helix and NEMO-binding domain, aa541-757, are inserted into pBHA and used as baites to screen a cDNA library, to express GST-fusion proteins in Escherichia coli, the gene fragments aa1-300, aa301-540, and aa541-756 are inserted into pGEX4T-1 or pCDNA3HA, all IKKbeta constructs, full length, aa1-300, aa301-757, aa1-540, and aa301-540-deleted forms, and IKKalpha are inserted into pCMV2-FLAG plasmids

Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [IkappaB protein]	Mus musculus	-	ADP + [IkappaB phosphoprotein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
GST-fusion protein are purified with glutathione sepharose 4B	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [IkappaB protein]	-	Mus musculus	ADP + [IkappaB phosphoprotein]	-	?

Synonyms

Synonyms	Comment	Organism
IkappaB kinase	-	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mus musculus

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Release 2023.1 (January 2023)