Literature summary for 2.7.11.10 extracted from

Shaul, J.D.; Farina, A.; Huxford, T.
The human IKKbeta subunit kinase domain displays CK2-like phosphorylation specificity (2008), Biochem. Biophys. Res. Commun., 374, 592-597.

Search for more literature for 2.7.11.10

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in baculovirus-infected sf9 insect cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
DELTA395-756	mutant lacking the leucine zipper domain, the helix-loop-helix domain, the serine rich and the NEMO-binding motif are capable to catalyze phosphotransfer to the substrate protein IkappaBalpha, but they do so at positions outside Ser-32 and Ser-36 while leaving these two critical amino acids unmodified. Removal of the leucine zipper and helix-loop-helix regions converts IKKbeta to monomer	Homo sapiens
DELTA395-756D145N	mutant lacking the leucine zipper domain, the helix-loop-helix domain, the serine rich and the NEMO-binding motif and containing a Asp145Asn mutation does not display any kinase activity	Homo sapiens
DELTA665-756	mutant lacking the serine rich and the NEMO-binding motif retains the ability to phosphorylate its substrate IkappaBalpha exclusively at serine positions 32 and 36	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O14920	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + IkappaBalpha	phosphorylation at Ser-32 and Ser-36	Homo sapiens	ADP + phosphorylated IkappaBalpha	-	?

Subunits

Subunits	Comment	Organism
dimer	gel filtration	Homo sapiens

Synonyms

Synonyms	Comment	Organism
IKKbeta	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	assay caried out at room temperature	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)