Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.1 extracted from

  • Zorina, A.; Bedbenov, V.; Novikova, G.; Panichkin, V.; Los, D.
    Involvement of serine/threonine protein kinases in the cold stress response in the cyanobacterium Synechocystis sp. PCC 6803 functional characterization of SpkE protein kinase (2014), Mol. Biol., 48, 390-398 .
No PubMed abstract available
Search for more literature for 2.7.11.1

Cloned(Commentary)

Cloned (Comment) Organism
gene slr1443, cloning by RT-PCR, recombinant expression of N-terminally GST-tagged in Escherichia coli strain Rosetta (DE3 pLysS) Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of a SpkE knockout DELTASpkE mutant strain, polypeptide profile and phosphorylation of individual proteins in the wild-type strain and the DELTASpkE mutant, phenotype, overview. The DELTASpkE protein pattern lacks several protein groups, including proteins of 65-75 kDa with pI 5.0-5.1, proteins of 65-75 kDa and a pI of about 5.3, and proteins of 45-50 kDa with pI 5.5 Synechocystis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information SpkE does not possess transmembrane domains Synechocystis sp.
-
-
soluble
-
 Synechocystis sp.
-
-

Organism

Organism UniProt Comment Textmining
Synechocystis sp. P73515 strain GS
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information SpkE activation does not seem to require phosphorylation of the enzyme Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone H1
-
 Synechocystis sp. ADP + phospho-histone H1
-
 ?
additional information recombinant GST-tagged SpkE does not display autophosphorylation activity, but is enzymatically active toward exogenous substrates. Recombinant GroES and myelin basic protein are no substrates for SpkE Synechocystis sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
serine/threonine-protein kinase-like protein E
-
 Synechocystis sp.
slr1443
-
 Synechocystis sp.
SpkE
-
 Synechocystis sp.

General Information

General Information Comment Organism
malfunction induction of ndhD2 transcription is dramatically decreased in the DELTASpk? mutant in both normal growth conditions and in stress. The decrease is significant in comparisons with wild-type GS cells and the cold stress sensor-defective Hik33 mutant. Transcription of gene crhR in the DELTASpkE mutant is somewhat lower than in wild-type cells in both optimal conditions and stress. The crhR transcription level in the DELTASpkE mutant is comparable with or slightly lower than in the DELTAHik33 mutant. Since crhR is partly controlled by Hik33, the finding may indirectly implicate SpkE in regulating crhR transcription. Transcription of desB is partly controlled by Hik33. desB mRNA accumulation in cold stress depends on the function of spkB, spkD, and spkE. The desB mRNA levels in the corresponding mutants are lower than in wild-type cells, comparable with or somewhat lower than in the DELTAHik33 mutant. Hik33 and SpkE act together to regulate desB expression. The transcription profile of the cold stress response genes in the SpkE mutant is similar to that of the Hik33 mutant Synechocystis sp.
physiological function SpkE is a functionally active protein kinase and utilizes basic cell proteins as preferential substrates. SpkE may act as an additional regulator of ndhD2 expression. The functional enzyme SpkE proves essential for phosphorylation of proteins with 50-75 kDa, and pI 5.6-5.8. SpkE may modify different isoforms of one protein. The enzyme SpkE is involved in transcriptional regulation during cold stress. None of the STPKs possessing transmembrane domains is involved in regulating the expression of the cold-inducible genes hliB, hliC, ndhD2, rbpA, and crhR, while enzymes SpkB, SpkD, SpkE, and SpkG act as possible regulators of the cold stress response occurring at the transcriptional level Synechocystis sp.