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Literature summary for 2.7.11.1 extracted from
- Identification and characterization of chloroplast casein kinase II from Oryza sativa (rice) (2015), J. Exp. Bot., 66, 175-187 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| polylysine | poly-lysine has a substrate-specific inhibitory effect on the CKII alpha subunit, whereas it stimulates the activity of the CKII holoenzyme | Oryza sativa |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| loc os03g55490, DNA and amino acid sequence determination and analysis, sequence comparisons, transient expression of GFP fused to the 184 N-terminal amino acids of the OspCKII sequence in Oryza sativa, recombinant expression of CKIIalpha without chloroplast transit peptide in Escherichia coli strain BL21 | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibition | Oryza sativa | |
| heparin | - |
Oryza sativa | |
| polylysine | poly-lysine has a substrate-specific inhibitory effect on the CKII alpha subunit, whereas it stimulates the activity of the CKII holoenzyme. Polylysine inhibits RNP29 phosphorylation by CKIIalpha | Oryza sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | transient expression of GFP fused to the 184 N-terminal amino acids of the OspCKII sequence in rice confirmed the chloroplastic localization of the kinase, subcellular localization analysis. Plastid CKII has a very low abundance. The N-terminal extension of LOC_Os03g55490 functions as a plastid transit peptide in vivo | Oryza sativa | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, activates | Oryza sativa | |
| Mn2+ | activates | Oryza sativa | |
| additional information | the phosphorylation activity is dependent on the presence of divalent ions such as Mg2+ or Mn2+ | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + casein | Oryza sativa | high activity | ADP + phospho-casein | - |
? | |
| additional information | Oryza sativa | rice leaf phosphoproteome analysis, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | Q8W5G9 | cv. japonica | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protoplast and chloroplast preparation from rice seedlings, purification of the enzyme from chloroplasts by heparin affinity chromatography | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Oryza sativa | - |
| seedling | - |
Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + casein | high activity | Oryza sativa | ADP + phospho-casein | - |
? | |
| ATP + RNP29 | recombinant Strep-tagged substrate expressed from Escherichia coli, phosphorylation of the canonical pCKII motif ESEGE in the model substrate, identification of the RNP29 phosphorylation site by peptide mapping and mass spectrometry | Oryza sativa | ADP + phospho-RNP29 | - |
? | |
| GTP + RNP29 | recombinant Strep-tagged substrate expressed from Escherichia coli, phosphorylation of the canonical pCKII motif ESEGE in the model substrate, identification of the RNP29 phosphorylation site by peptide mapping and mass spectrometry | Oryza sativa | GDP + phospho-RNP29 | - |
? | |
| additional information | rice leaf phosphoproteome analysis, overview | Oryza sativa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | chloroplast CKIIs lack the alpha'- and the beta-subunits ad are formed by alpha-subunits only. Oryza sativa contains four CKII alpha subunits with high sequence identity, peptide mapping and mass spectrometry, overview | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| casein kinase II | - |
Oryza sativa |
| CKII alpha | - |
Oryza sativa |
| LOC_Os03g55490 | locus name | Oryza sativa |
| OspCKII | - |
Oryza sativa |
| plastid casein kinase II | - |
Oryza sativa |
| plastid transcription kinase | - |
Oryza sativa |
| PTK | - |
Oryza sativa |
| RNP kinase | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Oryza sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Oryza sativa | |
| GTP | - |
Oryza sativa | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | several pCKII phosphorylation sites in dicotyledonous plants are not conserved in monocots and algae, suggesting that details of pCKII regulation in plastids have changed during evolution | Oryza sativa |
| physiological function | the plastid casein kinase II is part of the plastid RNA polymerase multisubunit enzyme complex as one of the associated regulatory factors that integrate the transcription machinery into different chloroplast processes. pCKII mediates transcriptional regulation by phosphorylation of RNA polymerase/TAC subunits | Oryza sativa |
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