Any feedback?
Literature summary for 2.7.11.1 extracted from
- Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole (2013), Mol. Cell. Biol., 33, 526-542.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ENV7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His6-tagged Env7 in Escherichia coli strain BL21(DE3)(pLysS), functional expression of HA- and GFP-tagged Env7 and overexpression of wild-type untagged Env7 in yeast cells. HA- and GFP-tagged Env7 localize to vacuolar membranes, and | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C13S/C14S/C15S | site-directed mutagenesis | Saccharomyces cerevisiae |
| E269A | site-directed mutagenesis, the mutant is degraded in a proteasome-dependent manner, its kinase function is abolished when stabilized | Saccharomyces cerevisiae |
| E269D | site-directed mutagenesis | Saccharomyces cerevisiae |
| E269S | site-directed mutagenesis | Saccharomyces cerevisiae |
| K69R/K70M | site-directed mutagenesis | Saccharomyces cerevisiae |
| K69R/K70R | site-directed mutagenesis | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endosome | at late endosome and vacuole interface | Saccharomyces cerevisiae | 5768 | - |
| Golgi apparatus | excessive overexpression of GFP-tagged Env7 leads to additional localization to the Golgi apparatus | Saccharomyces cerevisiae | 5794 | - |
| membrane | the enzyme's N-terminal Cys13 to -15 track is essential for Env7 vacuolar membrane anchoring and kinase activity | Saccharomyces cerevisiae | 16020 | - |
| vacuole | at late endosome and vacuole interface, GFP- and HA-tagged recombinant Env7s are vacuolar membrane proteins | Saccharomyces cerevisiae | 5773 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Saccharomyces cerevisiae |  |
| Mn2+ | can substitute for Mg2+ | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, about, His6-tagged enzyme, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
gene ENV7 | - |
| Saccharomyces cerevisiae BY4742 | - |
gene ENV7 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| lipoprotein | Env7 is a palmitoylated protein kinase | Saccharomyces cerevisiae |
| phosphoprotein | the enzyme performs autophosphorylation, also recombinant HA- and His-tagged Env7 proteins, and immunopreciptated HA-tagged Env7, are autophosphorylated in vitro | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged Env7 from Escherichia coli strain BL21(DE3)(pLysS) by nickel affinity chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-casein | - |
Saccharomyces cerevisiae | ADP + phosphorylated beta-casein | - |
? | |
| ATP + beta-casein | - |
Saccharomyces cerevisiae BY4742 | ADP + phosphorylated beta-casein | - |
? | |
| ATP + histone H1 | - |
Saccharomyces cerevisiae | ADP + phosphorylated histone H1 | - |
? | |
| ATP + histone H1 | - |
Saccharomyces cerevisiae BY4742 | ADP + phosphorylated histone H1 | - |
? | |
| ATP + histone H2B | - |
Saccharomyces cerevisiae | ADP + phosphorylated histone H2B | - |
? | |
| ATP + histone H2B | - |
Saccharomyces cerevisiae BY4742 | ADP + phosphorylated histone H2B | - |
? | |
| ATP + histone H3 | - |
Saccharomyces cerevisiae | ADP + phosphorylated histone H3 | - |
? | |
| ATP + histone H3 | - |
Saccharomyces cerevisiae BY4742 | ADP + phosphorylated histone H3 | - |
? | |
| ATP + myelin basic protein | - |
Saccharomyces cerevisiae | ADP + phosphorylated myelin basic protein | - |
? | |
| ATP + myelin basic protein | - |
Saccharomyces cerevisiae BY4742 | ADP + phosphorylated myelin basic protein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, about, His6-tagged enzyme, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Env7 | - |
Saccharomyces cerevisiae |
| serine/threonine kinase | - |
Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | gene ENV7 is a widely conserved orthologue of the serine/threonine kinase 16 (STK16) gene, phylogenetic anaysis | Saccharomyces cerevisiae |
| malfunction | ENV7 deletion leads to internal accumulation of precursor carboxypeptidase Y, a vacuolar hydrolytic enzyme that transits the rough endoplasmic reticulum, Golgi apparatus, and late endosome prior to arrival at the vacuole, where it is processed to mature form via PEP4-dependent proteolytic cleavage. env7DELTA is defective in maintaining fragmented vacuoles during hyperosmotic response and in buds | Saccharomyces cerevisiae |
| additional information | the enzyme's N-terminal Cys13 to -15 track is essential for Env7 vacuolar membrane anchoring and kinase activity | Saccharomyces cerevisiae |
| physiological function | possible role for STK16-related kinases in regulation of the endomembrane architecture. Env7 negatively regulates fusion at the vacuole membrane | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
