Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | autphosphorylation of Tssk4 at Thr197 is essential to its kinase activity | Mus musculus |
Cloned (Comment) | Organism |
---|---|
isozyme Tssk4, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged wild-type isozyme Tssk4 and of Myc-Tssk4T197A mutant in HEK-293T cells | Mus musculus |
isozymes Tssk1, Tssk2, and Tssk3, DNA and amino acid sequence determination and analysis, recombinant expression of the isozymes in HEK-293T cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
T197A | site-directed mutagenesis of the autophosphorylation site Thr residue of isozyme Tssk4 | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus | A3QQQ9 | isozyme Tssk4; isozyme Tssk4, is also named Tssk5 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Mus musculus |
phosphoprotein | autophosphorylation of Tssk4 at Th197 in the T-loop region is essential to its kinase activity | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type Tssk4 and Myc-Tssk4T197A mutant from HEK-293T cells by glutathione affinity and immunoaffinity chromatography, respectively. Native Tssk4 from testis gland by immunoaffinity precipitation | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Tssk4 is constantly expressed in the whole process of spermiogenesis | Mus musculus | - |
testis | - |
Mus musculus | - |
testis | in testis, mouse Tssk4 has four alternatively splicing variants at the transcriptional level | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Tssk4 has autophosphorylation activity and self-association character in vitro. Autophosphorylation of Tssk4 at Thr197 in the T-loop region is essential to its kinase activity | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
testis specific serine/threonine kinase 4 | - |
Mus musculus |
TSSK1 | - |
Mus musculus |
TSSK2 | - |
Mus musculus |
Tssk3 | - |
Mus musculus |
Tssk4 | - |
Mus musculus |
TSSK5 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the testis specific serine/threonine kinase family | Mus musculus |
additional information | self-association of Tssk4 | Mus musculus |
physiological function | Tssk4 kinase activity might be involved in spermiogenesis, Tssk4 is constantly expressed in the whole process of spermiogenesis. The autophosphorylation of Tssk4 at Thr197 is essential to its kinase activity | Mus musculus |