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Literature summary for 2.7.11.1 extracted from

  • Wei, Y.; Wang, X.; Fu, G.; Yu, L.
    Testis specific serine/threonine kinase 4 (Tssk4) maintains its kinase activity by phosphorylating itself at Thr-197 (2013), Mol. Biol. Rep., 40, 439-447.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information autphosphorylation of Tssk4 at Thr197 is essential to its kinase activity Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
isozyme Tssk4, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged wild-type isozyme Tssk4 and of Myc-Tssk4T197A mutant in HEK-293T cells Mus musculus
isozymes Tssk1, Tssk2, and Tssk3, DNA and amino acid sequence determination and analysis, recombinant expression of the isozymes in HEK-293T cells Mus musculus

Protein Variants

Protein Variants Comment Organism
T197A site-directed mutagenesis of the autophosphorylation site Thr residue of isozyme Tssk4 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Mus musculus A3QQQ9 isozyme Tssk4; isozyme Tssk4, is also named Tssk5
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
Mus musculus
phosphoprotein autophosphorylation of Tssk4 at Th197 in the T-loop region is essential to its kinase activity Mus musculus

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type Tssk4 and Myc-Tssk4T197A mutant from HEK-293T cells by glutathione affinity and immunoaffinity chromatography, respectively. Native Tssk4 from testis gland by immunoaffinity precipitation Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
additional information Tssk4 is constantly expressed in the whole process of spermiogenesis Mus musculus
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testis
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Mus musculus
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testis in testis, mouse Tssk4 has four alternatively splicing variants at the transcriptional level Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Tssk4 has autophosphorylation activity and self-association character in vitro. Autophosphorylation of Tssk4 at Thr197 in the T-loop region is essential to its kinase activity Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
testis specific serine/threonine kinase 4
-
Mus musculus
TSSK1
-
Mus musculus
TSSK2
-
Mus musculus
Tssk3
-
Mus musculus
Tssk4
-
Mus musculus
TSSK5
-
Mus musculus

General Information

General Information Comment Organism
evolution the enzyme is a member of the testis specific serine/threonine kinase family Mus musculus
additional information self-association of Tssk4 Mus musculus
physiological function Tssk4 kinase activity might be involved in spermiogenesis, Tssk4 is constantly expressed in the whole process of spermiogenesis. The autophosphorylation of Tssk4 at Thr197 is essential to its kinase activity Mus musculus