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Literature summary for 2.7.11.1 extracted from

  • Lower, B.H.; Bischoff, K.M.; Kennelly, P.J.
    The archaeon Sulfolobus solfataricus contains a membrane-associated protein kinase activity that preferentially phosphorylates threonine residues in vitro (2000), J. Bacteriol., 182, 3452-3459.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine i.e. ML-9, 0.5-1.0 mM, 50% inhibition Saccharolobus solfataricus
1-(5-isoquinolinylsulfonyl)-2-methylpiperazine i.e. H-7 Saccharolobus solfataricus
genistein
-
Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00074
-
ATP pH 6.5, 25°C Saccharolobus solfataricus
0.013
-
KKRAARATSNVFA pH 6.5, 25°C, presence of poly(Glu4:Tyr) Saccharolobus solfataricus
0.0157
-
ATP pH 6.5, 65°C Saccharolobus solfataricus
0.02
-
KKRAARASSNVFA pH 6.5, 65°C Saccharolobus solfataricus
0.03
-
KKRAARATSNVFA pH 6.5, 25°C Saccharolobus solfataricus
0.045
-
KKRAARATSNVFA pH 6.5, 65°C Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Saccharolobus solfataricus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ potential divalent metal ion cofactors Mn2+ and Mg2+ Saccharolobus solfataricus
Mn2+ potential divalent metal ion cofactors Mn2+ and Mg2+ Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
2 * 67000, SDS-PAGE Saccharolobus solfataricus
125000
-
gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the protein kinase undergoes autophosphorylation on threonine Saccharolobus solfataricus

Purification (Commentary)

Purification (Comment) Organism
partial Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0024
-
pH 6.5, 25°C, substrate: casein Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + casein phosphorylated on threonine residues Saccharolobus solfataricus AMP + phosphorylated casein
-
?
ATP + casein phosphorylated on threonine residues Saccharolobus solfataricus ADP + phosphorylated casein
-
?
ATP + histone H4 phosphorylated on threonine and on serine residues Saccharolobus solfataricus ADP + phosphorylated histone H4
-
?
ATP + KKRAARASSNVFA phosphorylation of the peptide is undetectable at 25°C. Phosphorylation takes place at measurable rates if the assay temperature is raised to 65°C or if poly(Glu4:Tyr) is included in the assay mixture Saccharolobus solfataricus ADP + ?
-
?
ATP + KKRAARATSNVFA
-
Saccharolobus solfataricus ADP + KKRAARApTSNVFA
-
?
ATP + reduced carboxyamidomethylated and maleylated lysozyme phosphorylated on threonine residues Saccharolobus solfataricus ADP + phosphorylated reduced carboxyamidomethylated and maleylated lysozyme
-
?
GDP + casein phosphorylated on threonine residues Saccharolobus solfataricus GMP + phosphorylated casein
-
?
GTP + casein phosphorylated on threonine residues Saccharolobus solfataricus GDP + phosphorylated casein
-
?
additional information neither of the pyrimidine nucleotide triphosphates tested, CTP and UTP, serves as substrates and neither does AMP, GMP, or pyrophosphate Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 67000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Saccharolobus solfataricus
65
-
assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Saccharolobus solfataricus