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Literature summary for 2.7.11.1 extracted from

  • Urabe, H.; Aoyagi, N.; Ogawara, H.; Motojima, K.
    Expression and characterization of the Streptomyces coelicolor serine/threonine protein kinase PkaD (2008), Biosci. Biotechnol. Biochem., 72, 778-785.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
K42N in vitro phosphorylation assay shows that mutant protein is not able to phosphorylate itself Streptomyces coelicolor
K42R in vitro phosphorylation assay shows that mutant protein is not able to phosphorylate itself Streptomyces coelicolor
additional information disruption of chromosomal pkaD results in a significant loss of actinorhodin production. This result implies the involvement of pkaD in the regulation of secondary metabolism Streptomyces coelicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane a hydrophobicity plot indicates the presence of a putative transmembrane spanning sequence downstream of the catalytic domain, suggesting that PkaD is a transmembrane protein kinase Streptomyces coelicolor 16020
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63900
-
deduced from cDNA Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor O83032
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-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein PkaD is found to be phosphorylated by itsself at the threonine and tyrosine residues Streptomyces coelicolor

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
PkaD
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Streptomyces coelicolor
serine-threonine kinase
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Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Streptomyces coelicolor