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Literature summary for 2.7.11.1 extracted from
- US3 of herpes simplex virus type 1 encodes a promiscuous protein kinase that phosphorylates and alters localization of lamin A/C in infected cells (2007), J. Virol., 81, 6459-6470.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-tagged enzyme | Human alphaherpesvirus 1 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Human alphaherpesvirus 1 |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + lamin A/C | Human alphaherpesvirus 1 | phosphorylation of host cell lamin by US3 kinase | ADP + phosphorylated lamin A/C | - |
? | |
| additional information | Human alphaherpesvirus 1 | the enzyme alters localization of lamin A/C in infected cells, recombinantly expressed lamin as GFP-tagged protein in Hep-G2 cells, enzyme inactivation causes capsids to aggregate aberrantly between the inner and outer nuclear membranes within evaginations/extensions of the perinuclear space, overview, US3 kinase activity regulates HSV-1 capsid nuclear regress at least in part by phosphorylation of lamin A/C | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Human alphaherpesvirus 1 | - |
HSV-1, gene US3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme by glutathione affinity chromatography | Human alphaherpesvirus 1 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
lamin disassembly assay, overview | Human alphaherpesvirus 1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + lamin A/C | phosphorylation of host cell lamin by US3 kinase | Human alphaherpesvirus 1 | ADP + phosphorylated lamin A/C | - |
? | |
| ATP + lamin A/C | phosphorylation of host cell lamin by US3 kinase at multiple sites in vitro, eventhough lamin A/C contains only one putative consensus sequence | Human alphaherpesvirus 1 | ADP + phosphorylated lamin A/C | - |
? | |
| additional information | the enzyme alters localization of lamin A/C in infected cells, recombinantly expressed lamin as GFP-tagged protein in Hep-G2 cells, enzyme inactivation causes capsids to aggregate aberrantly between the inner and outer nuclear membranes within evaginations/extensions of the perinuclear space, overview, US3 kinase activity regulates HSV-1 capsid nuclear regress at least in part by phosphorylation of lamin A/C | Human alphaherpesvirus 1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| US3 kinase | - |
Human alphaherpesvirus 1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Human alphaherpesvirus 1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Human alphaherpesvirus 1 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Human alphaherpesvirus 1 | |
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