Cloned (Comment) | Organism |
---|---|
expression of Rio2 in Escherichia coli | Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeled Rio2, hanging drop vapour diffusion method, equal volumes of protein solution, containing 5-12% PEG 900, and 0.1 M phosphate citrate buffer, pH 3.6-4.1, and reservoir solution, versus 1 ml reservoir solution, purified recombinant Rio2 complexed with ATP using 0.1 M Tris, pH 7.5, 20 mM ATP, 20 mM MgCl2, 20 mM adenosine 5'-(beta,gamma-imino)triphosphate, and 20% ethylene glycol, 20°C, 2-4 days, X-ray diffraction structure determination and analysis at 2.0 A resolution | Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Archaeoglobus fulgidus | kinase Rio2 is required for rRNA cleavage in 40S ribosomal subunit maturation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O30245 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | Rio2 performs autophosphorylation | Archaeoglobus fulgidus |
Purification (Comment) | Organism |
---|---|
recombinant Rio2 from Escherichia coli by adsorption chromatography and gel filtration | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-casein | dephosphorylated alpha-casein | Archaeoglobus fulgidus | ADP + phosphorylated alpha-casein | - |
? | |
ATP + histone | - |
Archaeoglobus fulgidus | ADP + phosphorylated histone | - |
? | |
ATP + myelin basic protein | - |
Archaeoglobus fulgidus | ADP + phosphorylated myelin basic protein | - |
? | |
additional information | kinase Rio2 is required for rRNA cleavage in 40S ribosomal subunit maturation | Archaeoglobus fulgidus | ? | - |
? | |
additional information | Rio2 performs autophosphorylation | Archaeoglobus fulgidus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the kinase domain is tightly connected to a wHTH domain | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the Rio family, Rio2 subfamily, of serine/threonine protein kinases | Archaeoglobus fulgidus |
Rio2 | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Archaeoglobus fulgidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding structure involving residues K168, S53, and D166, overview | Archaeoglobus fulgidus |