Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis of protein kinase PK1, expression as GST-fusion protein in Escherichia coli strain BL21 | Nicotiana tabacum |
DNA and amino acid sequence determination and analysis of protein kinase PK2, expression as GST-fusion protein in Escherichia coli strain BL21 | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Nicotiana tabacum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Nicotiana tabacum | |
Mn2+ | - |
Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39200 | - |
x * 39200, PK1, calculated from amino acid sequence | Nicotiana tabacum |
39500 | - |
x * 39500, PK2, calculated from amino acid sequence | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q709M0 | cv. Petit Havana | - |
Nicotiana tabacum | Q709M1 | cv. Petit Havana | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation of PK1 | Nicotiana tabacum |
phosphoprotein | autophosphorylation of PK2 | Nicotiana tabacum |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion PK1 from Escherichia coli strain BL21 by glutathione affinity chromatography, the GST-tag is cleaved off | Nicotiana tabacum |
recombinant GST-fusion PK2 from Escherichia coli strain BL21 by glutathione affinity chromatography, the GST-tag is cleaved off | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | developmental expression, overview | Nicotiana tabacum | - |
additional information | PK1 is not expressed in other tissues, developmental expression in pollen, overview | Nicotiana tabacum | - |
petal | - |
Nicotiana tabacum | - |
pistil | - |
Nicotiana tabacum | - |
pollen | - |
Nicotiana tabacum | - |
pollen tube | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PK1 performs autophosphorylation | Nicotiana tabacum | ? | - |
? | |
additional information | PK2 performs autophosphorylation | Nicotiana tabacum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39200, PK1, calculated from amino acid sequence | Nicotiana tabacum |
? | x * 39500, PK2, calculated from amino acid sequence | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
PK1 | - |
Nicotiana tabacum |
PK2 | - |
Nicotiana tabacum |
serine/threonine-specific protein kinase | - |
Nicotiana tabacum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Nicotiana tabacum |