Cloned (Comment) | Organism |
---|---|
expression of GST-fusion LOV2 and kinase domain LV2-KD of phototropin in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the chromophoric domain light-oxygen-voltage-sensing 2 LOV2 of phototropin acts as an intramolecular inhibitor to substrate protein phosphorylation | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | photoregulation of phototropin, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phototropin performs autophosphorylation | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion LOV2 and kinase domain LV2-KD of phototropin from Escherichia coli by glutathione affinity chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | the chromophoric domain light-oxygen-voltage-sensing 2 LOV2 of phototropin acts as a blue light-regulated molecular switch | Arabidopsis thaliana | ADP + phosphorylated casein | - |
? | |
additional information | photoregulation of phototropin, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | enzyme assay under dim red light conditions, phototropin performs autophosphorylation | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | phototropin has 2 chromophoric domains, the light-oxygen-voltage-sensing 1 and 2, i.e. LOV1 and LOV2, in the N-terminal half, and a Ser/Thr kinase motif in the C-terminal half | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
Phototropin | - |
Arabidopsis thaliana |
Ser/Thr kinase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |