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Literature summary for 2.7.11.1 extracted from

  • Zhang, Z.G.; Zhou, H.L.; Chen, T.; Gong, Y.; Cao, W.H.; Wang, Y.J.; Zhang, J.S.; Chen, S.Y.
    Evidence for serine/threonine and histidine kinase activity in the tobacco ethylene receptor protein NTHK2 (2004), Plant Physiol., 136, 2971-2981.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.1

Activating Compound

Activating Compound Comment Organism Structure
additional information NTHK2 is an inducible enzyme Nicotiana tabacum

Cloned(Commentary)

Cloned (Comment) Organism
NTHK2 DNA and amino acid sequence determination and analysis, expression of GST-fusion in Escherichia coli, expression of full-length and truncated NTHK2 kinase wild-type and mutants in Schizosaccharomyces pombe strain SP-Q01 Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
H384Q site-directed mutagenesis, the mutant cannot perform autophosphorylation of the receiver domain, but still phosphorylates its kinase domain Nicotiana tabacum
additional information construction of several NTHK2 kinase truncation mutants, overview Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can substitute for Mn2+ in serine/threonine kinase activity Nicotiana tabacum
Mn2+ dependent on, required for serine/threonine kinase activity Nicotiana tabacum
additional information the enzyme shows Mn2+-dependent serine/threonine kinase and Ca2+-dependent histidine kinase, EC 2.7.13.3, activities Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Nicotiana tabacum NTHK2 is an ethylene receptor protein ?
-
 ?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q9SDY2 NTHK2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the NTHK2 kinase performs serine/threonine autophosphorylation Nicotiana tabacum

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion full-length and truncated NTHK2 kinase wild-type and mutants from Escherichia coli by glutathione affinity chromatography Nicotiana tabacum

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate the enzyme shows Mn2+-dependent serine/threonine kinase and Ca2+-dependent histidine kinase, EC 2.7.13.3, activities Nicotiana tabacum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NTHK2 is an ethylene receptor protein Nicotiana tabacum ?
-
 ?
additional information substrate specificity of full-length enzyme and truncated mutant enzymes, overview, NTHK2 kinase performs autophosphorylation, the enzyme shows Mn2+-dependent serine/threonine kinase and Ca2+-dependent histidine kinase, EC 2.7.13.3, activities Nicotiana tabacum ?
-
 ?

Synonyms

Synonyms Comment Organism
ethylene receptor protein NTHK2
-
 Nicotiana tabacum
NTHK2 kinase
-
 Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Nicotiana tabacum