Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | insulin induces the phosphorylation of TSSK3, TSSK3 activation by phosphoinositide-dependent kinase-1 PDK1-dependent signalling, phosphorylation at Thr168 activates | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant GST-fusion TSSK3s in Escherichia coli strain BL21, expression of Myc-tagged catalytic subunit of phosphoinositide-dependent kinase-1 in human 293T cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
K39R | site-directed mutagenesis, inactive mutant | Mus musculus |
S166A | site-directed mutagenesis, mutation of a T-loop residue, highly reduced kinase activity compared to the wild-type TSSK3, no autophosphorylation | Mus musculus |
S166D | site-directed mutagenesis, mutation of a T-loop residue, reduced kinase activity compared to the wild-type TSSK3, no autophosphorylation | Mus musculus |
S166G | site-directed mutagenesis, mutation of a T-loop residue, highly reduced kinase activity compared to the wild-type TSSK3, no autophosphorylation | Mus musculus |
T168A | site-directed mutagenesis, mutation of a T-loop residue, inactive TSSK3 mutant, no autophosphorylation | Mus musculus |
T168D | site-directed mutagenesis, mutation of a T-loop residue, unaltered kinase activity and reduced autophosphorylation compared to the wild-type TSSK3 | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.145 | - |
myelin basic protein | pH 7.4, 30°C, recombinant TSSK3 | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus | |
Mn2+ | preferred cation, optimal at 10 mM | Mus musculus | |
additional information | divalent cations are required for activity | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + testis-specific serine-threonine kinase 3 | Mus musculus | substrate of phosphoinositide-dependent kinase-1 leading to activation | ADP + phosphorylated testis-specific serine-threonine kinase 3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | TSSK3 performs autophosphorylation, TSSK3 is phosphorylated at Thr168 of the T-loop by recombinant Myc-tagged catalytic subunit of phosphoinositide-dependent kinase-1 leading to activation of TSSK3, TSSK3 can also be phosphorylated by cAMP-dependent protein kinase PKA, EC 2.7.11.11, but not by protein kinase B | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant GST-fusion TSSK3s by glutathione affinity chromatography, recombinant Myc-tagged catalytic subunit of phosphoinositide-dependent kinase-1 from human 293T cells | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | testis-specific serine-threonine kinase 3, i.e. TSSK3 | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | substrate of TSSK3 | Mus musculus | ADP + phosphorylated casein | - |
? | |
ATP + histone H1 | substrate of TSSK3 | Mus musculus | ADP + phosphorylated histone H1 | - |
? | |
ATP + myelin basic protein | substrate of TSSK3 | Mus musculus | ADP + phosphorylated myelin basic protein | - |
? | |
ATP + RRSSSY | peptide substrate of TSSK3 consisting of the consensus sequence | Mus musculus | ADP + RRSS-phosphoS-Y | - |
? | |
ATP + testis-specific serine-threonine kinase 3 | substrate of phosphoinositide-dependent kinase-1 leading to activation | Mus musculus | ADP + phosphorylated testis-specific serine-threonine kinase 3 | - |
? | |
ATP + testis-specific serine-threonine kinase 3 | substrate of recombinant Myc-tagged catalytic subunit of PDK1 leading to activation | Mus musculus | ADP + phosphorylated testis-specific serine-threonine kinase 3 | - |
? | |
additional information | TSSK3 performs autophosphorylation | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | TSSK3 contains a T-loop structure | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme TSSK3 belongs to the family of testis-specific serine-threonine kinases | Mus musculus |
PDK1 | - |
Mus musculus |
phosphoinositide-dependent kinase-1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
TSSK3, and phosphoinositide-dependent kinase-1 assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
TSSK3 | Mus musculus |
7.5 | - |
PDK1 assay at | Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.2 | TSSK3 shows a broad range pH-optimum | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on, cannot be substituted by other nucleotide triphosphates like CTP or GTP | Mus musculus |