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Literature summary for 2.7.11.1 extracted from
- HIPK2 regulates transforming growth factor-beta-induced c-Jun NH(2)-terminal kinase activation and apoptosis in human hepatoma cells (2003), Cancer Res., 63, 8271-8277.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of wild-type and mutant FLAG-tagged HIPK2 in 293T cells, coexpression of wild-type and mutant HIPK2 with HA-tagged JNK1 in 293 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K221A | site-directed mutagenesis of HIPK2, mutant shows reduced interaction and colocalization with Daxx | Homo sapiens |
| additional information | construction of HIPK2 knockout mutants in Hep-3B cells | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nuclear body | HIPK2 colocalizes with Daxx in promyelotic leukemia nuclear bodies | Homo sapiens | 16604 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Daxx | Homo sapiens | Daxx is a protein acting in TGF-beta-induced JNK activation and in apoptosis | ADP + phosphorylated Daxx | - |
? | |
| additional information | Homo sapiens | HIPK2 regulates transforming growth factor-beta-induced c-Jun NH(2)-terminal kinase activation and apoptosis in hepatoma cells, HIPK2 triggers promyelotic leukemia nuclear body disruption and release of Daxx | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEP-3B cell | - |
Homo sapiens | - |
| hepatoma cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Daxx | Daxx is a protein acting in TGF-beta-induced JNK activation and in apoptosis | Homo sapiens | ADP + phosphorylated Daxx | - |
? | |
| ATP + Daxx | recombinant Daxx expressed as GST-fusion protein in Escherichia coli | Homo sapiens | ADP + phosphorylated Daxx | - |
? | |
| additional information | HIPK2 regulates transforming growth factor-beta-induced c-Jun NH(2)-terminal kinase activation and apoptosis in hepatoma cells, HIPK2 triggers promyelotic leukemia nuclear body disruption and release of Daxx | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HIPK2 | - |
Homo sapiens |
| homeodomain-interacting protein kinase 2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at, in vitro | Homo sapiens |
| 37 | - |
assay at, in vivo | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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