Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | WKN4 needs to be activated for activity | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of GST-fusion WNK4 and WNK1 kinases in Escherichia coli strain BL21(DE3), expression of WNK4 and WNK1 kinases, and of the autoinhibitory domain of WNK4, residues 444-518 and 444-563, as well as the N-terminal domain 1-443 in HEK-293T cells | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | WKN4 possesses an autoinhibitory domain, residues 444-518, the isolated autoinhibitory domainin 1-443 inhibits WKN1 | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | WKN1 and WKN4 are involved in regulation of ion transport across diverse epithelia, WKN4 reduces the activity of Na-Cl-cotransporter activity and the potassium channel ROMK by reducing their appearance in the plasma membrane | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation of WKN1 and WKN4 | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion WNK4 and WNK1 kinases from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + histone | - |
Rattus norvegicus | ADP + phosphorylated histone | - |
? | |
additional information | WKN1 and WKN4 are involved in regulation of ion transport across diverse epithelia, WKN4 reduces the activity of Na-Cl-cotransporter activity and the potassium channel ROMK by reducing their appearance in the plasma membrane | Rattus norvegicus | ? | - |
? | |
additional information | WKN1 and WKN4 perform autophosphorylation | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
WNK1 kinase | - |
Rattus norvegicus |
WNK4 kinase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |