Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.1 extracted from

  • Jergil, B.; Dixon, G.H.
    Protamine kinase from rainbow trout testis. Partial purification and characterization (1970), J. Biol. Chem., 245, 425-434.
    View publication on PubMed
Search for more literature for 2.7.11.1

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation, can replace DTT Oncorhynchus mykiss
3',5'-AMP
-
 Oncorhynchus mykiss

General Stability

General Stability Organism
freeze-thawing inactivates Oncorhynchus mykiss

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ not Oncorhynchus mykiss
high ionic strength activation, e.g. NaCl, NH4Cl, KCl, sodium acetate, essential for maximal phosphate incorporation with protamine as substrate, histone phosphorylation is suppressed Oncorhynchus mykiss
Mg2+ requirement Oncorhynchus mykiss
Mn2+ can replace Mg2+ with 8% efficiency Oncorhynchus mykiss
additional information no activation by Ca2+ or Zn2+ Oncorhynchus mykiss

Organism

Organism UniProt Comment Textmining
Oncorhynchus mykiss
-
 rainbow trout
-

Purification (Commentary)

Purification (Comment) Organism
partial Oncorhynchus mykiss

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.07
-
-
 Oncorhynchus mykiss

Storage Stability

Storage Stability Organism
0-4°C, partially purified preparation, several weeks Oncorhynchus mykiss

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein poor substrate Oncorhynchus mykiss ?
-
 ?
ATP + histone fraction II slightly lysine-rich, from trout testes Oncorhynchus mykiss ?
-
 ?
ATP + protamine preferred substrate Oncorhynchus mykiss ADP + O-phosphoprotamine
-
 ?
ATP + protamine transfers terminal phosphoryl group from ATP into O-phosphoseryl linkages in the acceptor molecule Oncorhynchus mykiss ADP + O-phosphoprotamine
-
 ?
ATP + protamine no substrates are GTP Oncorhynchus mykiss ADP + O-phosphoprotamine
-
 ?
ATP + protamine no substrates are GTP or UTP Oncorhynchus mykiss ADP + O-phosphoprotamine
-
 ?
ATP + protamine phosphorylates histone fraction I (lysine-rich) and histone fractions III and IV (arginine-rich), from trout testes Oncorhynchus mykiss ADP + O-phosphoprotamine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Oncorhynchus mykiss

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8 at high salt concentration Oncorhynchus mykiss

pH Range

pH Minimum pH Maximum Comment Organism
6.4 8.6 about half-maximal activity at pH 6.4 and about 65% of maximal activity at pH 8.6, at high salt concentration Oncorhynchus mykiss