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Literature summary for 2.7.11.1 extracted from

  • Damuni, Z.; Reed, L.J.
    Purification and properties of a protamine kinase and a type II casein kinase from bovine kidney mitochondria (1988), Arch. Biochem. Biophys., 262, 574-584.
    View publication on PubMed

General Stability

General Stability Organism
diluted solutions are unstable Bos taurus
repeated freeze-thawing inactivates Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Bos taurus
additional information heparin, Ca2+/calmodulin, phosphatidylserine and/or diolein (in the absence or presence of Ca2+); protein inhibitor of cAMP-dependent protein kinase (protamine as substrate), monoclonal antibodies to catalytic domain of protein kinase C from rat brain Bos taurus
NaCl bovine serum albumin, not protamine as substrate Bos taurus
O-phosphoprotamine bovine serum albumin, not protamine as substrate Bos taurus
spermine bovine serum albumin, not protamine as substrate Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-
soluble
-
Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1.5 mM (mitochondrial kinase) Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mitochondrial kinase Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.11
-
-
Bos taurus

Storage Stability

Storage Stability Organism
-20°C, in 0.05 M imidazole chloride, pH 7.3, 10% glycerol, 1 mM DTT, protease inhibitors, 1 mM EDTA, at least 6 months Bos taurus
0-4°C, in 0.05 M imidazole chloride, pH 7.3, 10% glycerol, 1 mM DTT, protease inhibitors, 1 mM EDTA, up to 3 weeks Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + bovine serum albumin phosphorylated at 40-70% the rate of protamine Bos taurus ?
-
?
ATP + glycogen synthase phosphorylated at 15% the rate of protamine Bos taurus ?
-
?
ATP + histone H1 phosphorylated at 80% the rate of protamine Bos taurus ?
-
?
ATP + protamine preferred substrate Bos taurus ADP + O-phosphoprotamine
-
?
ATP + protamine phosphorylates little, if any activity with branched-chain alpha-keto acid dehydrogenase, pyruvate dehydrogenase, casein, ovalbumin or histone H2B Bos taurus ADP + O-phosphoprotamine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1 8.2 protamine as substrate Bos taurus
6.7
-
bovine serum albumin as substrate Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5.8 8.8 about 85% of maximal activity at pH 5.8 and about 80% of maximal activity at pH 8.8, protamine as substrate Bos taurus
6.6 6.8 about half-maximal activity at pH 6.6 and pH 6.8, bovine serum albumin as substrate Bos taurus