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Literature summary for 2.7.11.1 extracted from
- Protamine kinase from yeast (1975), Biochim. Biophys. Acta, 397, 117-123.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3',5'-AMP | not | Saccharomyces cerevisiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | above 30 mM, activating below 20 mM | Saccharomyces cerevisiae | |
| Mg2+ | above 30 mM, activating below 20 mM | Saccharomyces cerevisiae | |
| Mn2+ | above 30 mM, activating below 20 mM | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
ATP | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | requirement, 1-10 mM, can replace Mg2+ to some extent, inhibits above 30 mM | Saccharomyces cerevisiae | |
| Mg2+ | requirement | Saccharomyces cerevisiae | |
| Mg2+ | inhibits above 30 mM | Saccharomyces cerevisiae | |
| Mn2+ | can replace Mg2+ to some extent | Saccharomyces cerevisiae | |
| Mn2+ | inhibits above 30 mM | Saccharomyces cerevisiae | |
| Mn2+ | requirement, 1-10 mM | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, at least 2 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + protamine | phosphorylates serine | Saccharomyces cerevisiae | ADP + O-phosphoprotamine | - |
? | |
| ATP + protamine | no substrates are rabbit muscle glycogen phosphorylase b, human gamma-globulin | Saccharomyces cerevisiae | ADP + O-phosphoprotamine | - |
? | |
| ATP + protamine | no substrates are acidic proteins, e.g. casein, phosvitin | Saccharomyces cerevisiae | ADP + O-phosphoprotamine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
at pH 7.5, t1/2: 10 min | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
at 50°C, t1/2: 10 min | Saccharomyces cerevisiae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
5.9 | 5.6 |
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Release 2023.1 (January 2023)
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