Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.10.2 extracted from

  • Emmons, T.L.; Wrightstone, A.D.; Baima, E.T.; Brown, S.; Sommers, C.D.; Hirsch, J.L.; Pegg, L.E.; Weinberg, R.A.; Fischer, H.D.; Wittwer, A.J.; Tomasselli, A.G.
    Differential kinetics and inhibition of purified recombinant tyrosine kinase 2 (TYK-2) and its catalytic domain JH-1 (2012), Protein Pept. Lett., 19, 485-491.
    View publication on PubMed

Application

Application Comment Organism
synthesis expression and a two-step purification procedure for the doubly tagged full-length expression construct, H6-FL-TYK-2-FLAG. In the presence of ATP and a peptide substrate, H6-FL-TYK-2-FLAG shows a marked lag in phosphopeptide product formation, while the wild-type shows no such lag. The lag can be eliminated by ATP pretreatment. The potencies of several nanomolar inhibitors are similar for TYK-2 KD and H6-FL-TYK-2-FLAG. However, these same inhibitors are about 1000 times less potent inhibiting the autophosphorylation of H6-FL-TYK-2-FLAG than they are inhibiting the phosphorylation of a peptide substrate modeled after the activation loop sequence of wild-type TYK-2 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information expression and a two-step purification procedure for the doubly tagged full-length expression construct, H6-FL-TYK-2-FLAG. In the presence of ATP and a peptide substrate, H6-FL-TYK-2-FLAG shows a marked lag in phosphopeptide product formation, while the wild-type shows no such lag. The lag can be eliminated by ATP pretreatment. The potencies of several nanomolar inhibitors are similar for TYK-2 KD and H6-FL-TYK-2-FLAG. However, these same inhibitors are about 1000 times less potent inhibiting the autophosphorylation of H6-FL-TYK-2-FLAG than they are inhibiting the phosphorylation of a peptide substrate modeled after the activation loop sequence of wild-type TYK-2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P29597
-
-

Synonyms

Synonyms Comment Organism
TYK-2
-
Homo sapiens
tyrosine kinase 2
-
Homo sapiens