Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant Csk in Escherichia coli strain DH5 alpha | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D518A | site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
D518E | site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
D518G | site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
D518N | site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
E324A | site-directed mutagenesis of Csk, the mutant enzyme shows unaltered interaction with the Src substrate, and slightly reduced activity with the polyEY substrate | Homo sapiens |
E510A | site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
E510R | site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
K295M | a catalytically inactive mutant Src | Homo sapiens |
L296A | site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate, and reduced activity with the polyEY substrate | Homo sapiens |
N326A | site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate, and reduced activity with the polyEY substrate | Homo sapiens |
R279A | site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate | Homo sapiens |
R281A | site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate | Homo sapiens |
R283A | site-directed mutagenesis of Csk, the mutant enzyme shows highly reduced interaction with the Src substrate, and slightly reduced activity with the polyEY substrate | Homo sapiens |
R283A | site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate | Homo sapiens |
R283E | site-directed mutagenesis of Csk, the mutant enzyme shows highly reduced interaction with the Src substrate | Homo sapiens |
R283K | site-directed mutagenesis of Csk, the mutant enzyme shows highly reduced interaction with the Src substrate | Homo sapiens |
S280A | site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate | Homo sapiens |
Y511A | site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
Y511F | site-directed mutagenesis of Src, the mutant enzyme shows reduced slightly interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
Y511I | site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
Y511L | site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
Y511W | site-directed mutagenesis of Src, the mutant enzyme shows reduced slightly interaction with the Csk and reduced inactivation by Csk | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein tyrosine kinase Yes | Homo sapiens | Csk inactivates the enzyme substrate | ADP + phospho-L-tyrosinyl Yes | - |
? | |
ATP + Src family kinase | Homo sapiens | terminal Src kinase Csk specifically phosphorylates Src family kinases on a C-terminal Tyr residue, which down-regulates their activities, identification of the docking determinants in Src recognized by the Csk substrate-docking site | ADP + phospho-L-tyrosinyl Src family kinase | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + EEEEY | - |
Homo sapiens | ADP + EEEE(phospho)Y | - |
? | |
ATP + protein tyrosine kinase Yes | Csk inactivates the enzyme substrate | Homo sapiens | ADP + phospho-L-tyrosinyl Yes | - |
? | |
ATP + Src | wild-type Src and catalytically inactive mutant Src K295M, terminal Src kinase Csk specifically phosphorylates Src family kinases on a C-terminal Tyr residue, which down-regulates their activities, identification of the docking determinants in Src recognized by the Csk substrate-docking site, Glu510 of Src interacts with Arg283 of Csk in Csk-Src recognition, activity with Src fragments, overview | Homo sapiens | ADP + phospho-L-tyrosinyl Src family kinase | - |
? | |
ATP + Src family kinase | terminal Src kinase Csk specifically phosphorylates Src family kinases on a C-terminal Tyr residue, which down-regulates their activities, identification of the docking determinants in Src recognized by the Csk substrate-docking site | Homo sapiens | ADP + phospho-L-tyrosinyl Src family kinase | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C-terminal Src kinase | - |
Homo sapiens |
Csk | - |
Homo sapiens |
Protein tyrosine kinase | - |
Homo sapiens |
Src family kinase | - |
Homo sapiens |
Yes | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |