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Literature summary for 2.7.10.2 extracted from

  • Lee, S.; Ayrapetov, M.K.; Kemble, D.J.; Parang, K.; Sun, G.
    Docking-based substrate recognition by the catalytic domain of a protein tyrosine kinase, C-terminal Src kinase (Csk) (2006), J. Biol. Chem., 281, 8183-8189.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant Csk in Escherichia coli strain DH5 alpha Homo sapiens

Protein Variants

Protein Variants Comment Organism
D518A site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk Homo sapiens
D518E site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk Homo sapiens
D518G site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk Homo sapiens
D518N site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk Homo sapiens
E324A site-directed mutagenesis of Csk, the mutant enzyme shows unaltered interaction with the Src substrate, and slightly reduced activity with the polyEY substrate Homo sapiens
E510A site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk Homo sapiens
E510R site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk Homo sapiens
K295M a catalytically inactive mutant Src Homo sapiens
L296A site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate, and reduced activity with the polyEY substrate Homo sapiens
N326A site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate, and reduced activity with the polyEY substrate Homo sapiens
R279A site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate Homo sapiens
R281A site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate Homo sapiens
R283A site-directed mutagenesis of Csk, the mutant enzyme shows highly reduced interaction with the Src substrate, and slightly reduced activity with the polyEY substrate Homo sapiens
R283A site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate Homo sapiens
R283E site-directed mutagenesis of Csk, the mutant enzyme shows highly reduced interaction with the Src substrate Homo sapiens
R283K site-directed mutagenesis of Csk, the mutant enzyme shows highly reduced interaction with the Src substrate Homo sapiens
S280A site-directed mutagenesis of Csk, the mutant enzyme shows reduced interaction with the Src substrate Homo sapiens
Y511A site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk Homo sapiens
Y511F site-directed mutagenesis of Src, the mutant enzyme shows reduced slightly interaction with the Csk and reduced inactivation by Csk Homo sapiens
Y511I site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk Homo sapiens
Y511L site-directed mutagenesis of Src, the mutant enzyme shows reduced interaction with the Csk and reduced inactivation by Csk Homo sapiens
Y511W site-directed mutagenesis of Src, the mutant enzyme shows reduced slightly interaction with the Csk and reduced inactivation by Csk Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + protein tyrosine kinase Yes Homo sapiens Csk inactivates the enzyme substrate ADP + phospho-L-tyrosinyl Yes
-
?
ATP + Src family kinase Homo sapiens terminal Src kinase Csk specifically phosphorylates Src family kinases on a C-terminal Tyr residue, which down-regulates their activities, identification of the docking determinants in Src recognized by the Csk substrate-docking site ADP + phospho-L-tyrosinyl Src family kinase
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + EEEEY
-
Homo sapiens ADP + EEEE(phospho)Y
-
?
ATP + protein tyrosine kinase Yes Csk inactivates the enzyme substrate Homo sapiens ADP + phospho-L-tyrosinyl Yes
-
?
ATP + Src wild-type Src and catalytically inactive mutant Src K295M, terminal Src kinase Csk specifically phosphorylates Src family kinases on a C-terminal Tyr residue, which down-regulates their activities, identification of the docking determinants in Src recognized by the Csk substrate-docking site, Glu510 of Src interacts with Arg283 of Csk in Csk-Src recognition, activity with Src fragments, overview Homo sapiens ADP + phospho-L-tyrosinyl Src family kinase
-
?
ATP + Src family kinase terminal Src kinase Csk specifically phosphorylates Src family kinases on a C-terminal Tyr residue, which down-regulates their activities, identification of the docking determinants in Src recognized by the Csk substrate-docking site Homo sapiens ADP + phospho-L-tyrosinyl Src family kinase
-
?

Synonyms

Synonyms Comment Organism
C-terminal Src kinase
-
Homo sapiens
Csk
-
Homo sapiens
Protein tyrosine kinase
-
Homo sapiens
Src family kinase
-
Homo sapiens
Yes
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens