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Literature summary for 2.7.10.2 extracted from

  • Camara-Artigas, A.; Palencia, A.; Martinez, J.C.; Luque, I.; Gavira, J.A.; Garcia-Ruiz, J.M.
    Crystallization by capillary counter-diffusion and structure determination of the N114A mutant of the SH3 domain of Abl tyrosine kinase complexed with a high-affinity peptide ligand (2007), Acta Crystallogr. Sect. D, 63, 646-652.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and N114A mutant SH3 domain in Escherichia coli strain BL21 (DE3) Abelson leukaemia virus

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type and mutant Abl tyrosine kinase SH3 domains complexed with the high-affinity peptide p41 APSYSPPPPP, 8 mg/ml protein in 50 mM glycine buffer, pH 3.0, mixed in a molar protein:ligand ratio of 1:2 with lyophilized p41, method development with different precipitant solutions, optimally at pH 7.0, X-ray diffraction structure determination and analysis at 1.75 A resolution, molecular replacement Abelson leukaemia virus

Protein Variants

Protein Variants Comment Organism
N114A site-directed mutagenesis in the proline-rich region, mutant of the SH3 domain, structure determination in a complex with a proline-rich peptide, comparison to the wild-type enzyme, overview Abelson leukaemia virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information thermodynamics Abelson leukaemia virus

Organism

Organism UniProt Comment Textmining
Abelson leukaemia virus
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and N114A mutant SH3 domain from Escherichia coli strain BL21 (DE3) by ammonium sulfate fractionation and gel filtration Abelson leukaemia virus

Synonyms

Synonyms Comment Organism
Abl tyrosine kinase
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Abelson leukaemia virus