Application | Comment | Organism |
---|---|---|
analysis | development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
phylogenetic tree of kinases derived from the kinase core sequence, overview, expression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | Saccharomyces cerevisiae | - |
ADP + a phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme performs autophosphorylation | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | - |
Saccharomyces cerevisiae | ADP + a phosphoprotein | - |
? | |
ATP + poly-(Tyr-Glu) | tyrosine kinase substrate, phosphoryated by Swe1p, Mps1p, Ime2p, and Hrr25p, but not by Ste7p, Rad53p, or Mck1p | Saccharomyces cerevisiae | ADP + phospho-poly-(Tyr-Glu) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Hrr25p | - |
Saccharomyces cerevisiae |
Ime2p | - |
Saccharomyces cerevisiae |
Mps1p | - |
Saccharomyces cerevisiae |
Swe1p | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |