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Literature summary for 2.7.10.2 extracted from

  • Lin, X.; Ayrapetov, M.K.; Sun, G.
    Characterization of the interactions between the active site of a protein tyrosine kinase and a divalent metal activator (2005), BMC Biochem., 6, 25.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development PTKS are key targets for anticancer drug discovery Homo sapiens
pharmacology PTKS are key targets for anticancer drug discovery Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ essential, 2 Mg2+ are required, one Mg2+ binds to ATP as MgATP-, the other Mg2+ acts as an essential activator on the enzyme, whose binding is pH-sensitive between pH 6.0 and pH 7.0, M2 binding involves Glu236 and Asp332, binding structure, overview Homo sapiens
additional information the ability of metal cations to interact with Csk correlates with a combination of ionic radius and the coordination number Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a [protein]-L-tyrosine Homo sapiens
-
ADP + a [protein]-L-tyrosine phosphate
-
?
additional information Homo sapiens PTKs are involved in cell signalling ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate catalytic mechanism Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a [protein]-L-tyrosine
-
Homo sapiens ADP + a [protein]-L-tyrosine phosphate
-
?
ATP + poly(Glu4-Tyr) artificial substrate Homo sapiens ADP + poly(Glu4-Tyr)-L-tyrosine phosphate
-
?
ATP + [kdSrc kinase]-L-tyrosine i.e. kinase-defective chicken Src mutant K295M Homo sapiens ADP + [kdSrc kinase]-L-tyrosine phosphate
-
?
additional information PTKs are involved in cell signalling Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
C-terminal Src kinase
-
Homo sapiens
Csk
-
Homo sapiens
More Csk belongs to the Src kinase family Homo sapiens
Protein tyrosine kinase
-
Homo sapiens
PTK
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP as MgATP- Homo sapiens