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Literature summary for 2.7.10.1 extracted from

  • Gajadhar, A.; Guha, A.
    A proximity ligation assay using transiently transfected, epitope-tagged proteins: Application for in situ detection of dimerized receptor tyrosine kinases (2010), Biotechniques, 48, 145-152.
    View publication on PubMed

Application

Application Comment Organism
analysis proximity ligation assay-based methodology for in situ visualization and quantification of ligand-dependent EGFR receptor dimerization in intact cells. Using the approach combined with a universally applicable epitope tagging strategy, EGFR dimers can be detect in cells transiently co-expressing FLAG-tagged and MYC-tagged human EGFRs. Data strongly suggest that the method can be used to detect ligand-dependent EGFR dimerization, and the signal is generated in a protein interaction-based manner Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00533 EGFR
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