Cloned (Comment) | Organism |
---|---|
expression of a His-tagged enzyme fragment comprising residues 544-693 and 754-935, lacking the kinase insertion domain KID, in Spodoptera frugiperda cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme fragment, activated or autoinhibited, comprising residues 544-693 and 754-935, sitting drop vapour diffusion method, 0.005 ml active protein solution, containing 6 mg/ml protein 250 mM NaCl, MgCl2, and ATP, are mixed with 0.005 ml reservoir solution containing 18% polyethylene glycol 8000, 0.1 M MES, pH 7.1, 20°C, 50 nl autoinhibited protein solution is mixed with 50 nl reservoir solution containing 13% PEG 8000, 0.1 M HEPES, pH 7.0, co-crystallization with inhibitor STI-571 50 nl inhibitor solution and 50 nl reservoir solution containing 2 M phosphate, 0.1 M Tris, pH 8.5 are utilized, X-ray diffraction structure determination and analysis at about 1.38 A | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme performs autoinhibition via its juxtamembrane domain, structure and mechanism, overview | Homo sapiens | |
STI-571 | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | enzyme contains a juxtamembrane domain acting as a pseudosubstrate responsible for autoinhibition of the enzyme, structure overview | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | coordinates with Asp810 within the c-Kit DFG motif, overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P10721 | juxtamembrane domain and catalytic domains of c-Kit receptor protein-tyrosine kinase, residues 544-935 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme fragment comprising residues 544-693 and 754-935 by nickel chelate affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a [protein]-L-tyrosine | substrate binding and active structure | Homo sapiens | ADP + a [protein]-L-tyrosine phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
c-Kit receptor protein-tyrosine kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |