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Literature summary for 2.7.10.1 extracted from

  • Sheth, P.R.; Watowich, S.J.
    Oligomerization-induced differential dephosphorylation of c-Met receptor tyrosine kinase (2005), Biochemistry, 44, 10984-10993.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged cytoplasmic domain of c-Met receptor tyrosine kinase and a His-tagged transmembrane and cytoplasmic domain construct in Spodoptera frugiperda Sf9 cells via baculovirus infection system, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information enzyme dephosphorylation kinetics Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens
Mn2+
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
1 * 44000, recombinant cytoplasmic domain cytoMet of c-Met receptor tyrosine kinase, SDS-PAGE Homo sapiens
60000
-
2 * 60000, recombinant His-tagged transmembrane and cytoplasmic domain construct of c-Met receptor tyrosine kinase, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein enzyme performs autophosphorylation, ligand-dependent, activation by phosphorylation at tyrosine residues of the enzyme, enzyme oligomerization induced enzyme dephosphorylation by protein tyrosine phosphatase leading to inactivation of the protein tyrosine kinase, dephosphorylation kinetics, phosphorylation level of recombinant His-tagged enzyme domain constructs, overview Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme domain constructs from Sf9 insect cells by nickel affinity chromatography to about 90% homogeneity Homo sapiens

Subunits

Subunits Comment Organism
dimer 2 * 60000, recombinant His-tagged transmembrane and cytoplasmic domain construct of c-Met receptor tyrosine kinase, SDS-PAGE Homo sapiens
monomer 1 * 44000, recombinant cytoplasmic domain cytoMet of c-Met receptor tyrosine kinase, SDS-PAGE Homo sapiens
More domain organization, oligomerization induced enzyme dephosphorylation by protein tyrosine phosphatase leading to inactivation of the protein tyrosine kinase Homo sapiens

Synonyms

Synonyms Comment Organism
c-Met receptor tyrosine kinase
-
Homo sapiens
c-Met RTK
-
Homo sapiens