Cloned (Comment) | Organism |
---|---|
expression of His-tagged cytoplasmic domain of c-Met receptor tyrosine kinase and a His-tagged transmembrane and cytoplasmic domain construct in Spodoptera frugiperda Sf9 cells via baculovirus infection system, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme dephosphorylation kinetics | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
1 * 44000, recombinant cytoplasmic domain cytoMet of c-Met receptor tyrosine kinase, SDS-PAGE | Homo sapiens |
60000 | - |
2 * 60000, recombinant His-tagged transmembrane and cytoplasmic domain construct of c-Met receptor tyrosine kinase, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | enzyme performs autophosphorylation, ligand-dependent, activation by phosphorylation at tyrosine residues of the enzyme, enzyme oligomerization induced enzyme dephosphorylation by protein tyrosine phosphatase leading to inactivation of the protein tyrosine kinase, dephosphorylation kinetics, phosphorylation level of recombinant His-tagged enzyme domain constructs, overview | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme domain constructs from Sf9 insect cells by nickel affinity chromatography to about 90% homogeneity | Homo sapiens |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000, recombinant His-tagged transmembrane and cytoplasmic domain construct of c-Met receptor tyrosine kinase, SDS-PAGE | Homo sapiens |
monomer | 1 * 44000, recombinant cytoplasmic domain cytoMet of c-Met receptor tyrosine kinase, SDS-PAGE | Homo sapiens |
More | domain organization, oligomerization induced enzyme dephosphorylation by protein tyrosine phosphatase leading to inactivation of the protein tyrosine kinase | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
c-Met receptor tyrosine kinase | - |
Homo sapiens |
c-Met RTK | - |
Homo sapiens |