BRENDA - Enzyme Database show
show all sequences of 2.7.1.B28

Characterization of inductive synthesis of levoglucosan kinase by a combined strategy of enzymological and fast atom bombardment mass spectrometric analysis

Zhuang, X.L.; Zhang, H.X.; Sheng Wu Gong Cheng Xue Bao 5, 583-587 (2002)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Aspergillus niger
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
Aspergillus niger
direct formation of glucose 6-phosphate from levoglucosan in the presence of ATP and MgCl2
ADP + 6-phospho-alpha-D-glucopyranose
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
Aspergillus niger CBX-209
direct formation of glucose 6-phosphate from levoglucosan in the presence of ATP and MgCl2
ADP + 6-phospho-alpha-D-glucopyranose
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
-
-
-
Aspergillus niger CBX-209
-
-
-
Purification (Commentary)
Commentary
Organism
native enzymepartially by ammonium sulfate fractionation and ion exchange chromatography
Aspergillus niger
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
inductive synthesis of levoglucosan kinase, the organism grows well on levoglucosan as sole carbon source, fermenting it into citric acid with a yield comparable to that on glucose
Aspergillus niger
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
direct formation of glucose 6-phosphate from levoglucosan in the presence of ATP and MgCl2
739710
Aspergillus niger
ADP + 6-phospho-alpha-D-glucopyranose
-
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
direct formation of glucose 6-phosphate from levoglucosan in the presence of ATP and MgCl2
739710
Aspergillus niger CBX-209
ADP + 6-phospho-alpha-D-glucopyranose
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Aspergillus niger
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Aspergillus niger
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Aspergillus niger
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
Aspergillus niger
direct formation of glucose 6-phosphate from levoglucosan in the presence of ATP and MgCl2
ADP + 6-phospho-alpha-D-glucopyranose
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
Aspergillus niger CBX-209
direct formation of glucose 6-phosphate from levoglucosan in the presence of ATP and MgCl2
ADP + 6-phospho-alpha-D-glucopyranose
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzymepartially by ammonium sulfate fractionation and ion exchange chromatography
Aspergillus niger
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
inductive synthesis of levoglucosan kinase, the organism grows well on levoglucosan as sole carbon source, fermenting it into citric acid with a yield comparable to that on glucose
Aspergillus niger
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
direct formation of glucose 6-phosphate from levoglucosan in the presence of ATP and MgCl2
739710
Aspergillus niger
ADP + 6-phospho-alpha-D-glucopyranose
-
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
direct formation of glucose 6-phosphate from levoglucosan in the presence of ATP and MgCl2
739710
Aspergillus niger CBX-209
ADP + 6-phospho-alpha-D-glucopyranose
-
-
-
?
Other publictions for EC 2.7.1.B28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738435
Bacik
Bioconversion of anhydrosugars ...
Alternaria alternata, Aspergillus niger, Aspergillus niger CBX-209, Aspergillus terreus, Buckleyzyma aurantiaca, Hannaella luteola, Lipomyces starkeyi, Lipomyces starkeyi YZ-215, Papiliotrema flavescens, Papiliotrema laurentii, Penicillium herquei, Penicillium javanicum, Sporidiobolus salmonicolor
IUBMB Life
68
700-708
2016
-
-
2
-
1
-
4
22
-
12
-
13
-
16
-
-
2
2
-
-
-
1
18
-
2
-
1
-
2
-
2
11
1
-
3
-
-
2
11
-
1
-
3
4
1
22
-
12
-
13
-
-
-
2
-
-
-
1
18
-
2
-
1
-
2
-
2
-
-
1
1
-
-
-
738664
Bacik
Producing glucose 6-phosphate ...
Lipomyces starkeyi, Lipomyces starkeyi YZ-215
J. Biol. Chem.
290
26638-26648
2015
-
-
1
1
-
-
-
-
-
1
-
2
-
3
-
-
1
1
-
-
-
-
6
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
738937
Dai
Computational analysis of AnmK ...
Aspergillus terreus, Lipomyces starkeyi
J. Theor. Biol.
379
59-65
2015
-
-
2
-
-
-
-
-
-
2
-
2
-
7
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
738262
Xie
Screening and identification o ...
Aspergillus niger
FEMS Microbiol. Lett.
251
313-319
2005
-
-
1
-
-
-
-
-
-
1
1
1
-
6
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
739585
Zhuang
Identification, characterizati ...
Aspergillus niger, Aspergillus niger CBX-209
Protein Expr. Purif.
1
71-81
2002
-
-
1
-
-
-
1
2
-
1
1
2
-
8
-
-
1
-
-
-
-
1
6
1
1
-
1
-
1
-
1
1
1
-
-
-
-
1
1
-
-
-
-
1
1
2
-
1
1
2
-
-
-
1
-
-
-
1
6
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
739710
Zhuang
Characterization of inductive ...
Aspergillus niger, Aspergillus niger CBX-209
Sheng Wu Gong Cheng Xue Bao
5
583-587
2002
-
-
-
-
-
-
-
-
-
1
-
2
-
5
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-