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show all sequences of 2.7.1.B28

Identification, characterization of levoglucosan kinase, and cloning and expression of levoglucosan kinase cDNA from Aspergillus niger CBX-209 in Escherichia coli

Zhuang, X.; Zhang, H.; Protein Expr. Purif. 1, 71-81 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene lgk, recombinant expression in Escherichia coli strain DH5alpha
Aspergillus niger
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
competitive inhibition
Aspergillus niger
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
ATP
pH 9.3, 30°C
Aspergillus niger
71.2
-
1,6-anhydro-beta-D-glucopyranose
pH 9.3, 30°C
Aspergillus niger
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Aspergillus niger
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
x * 56000, SDS-PAGE
Aspergillus niger
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
Aspergillus niger
-
ADP + 6-phospho-alpha-D-glucopyranose
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
Aspergillus niger CBX-209
-
ADP + 6-phospho-alpha-D-glucopyranose
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
-
gene lgk
-
Aspergillus niger CBX-209
-
gene lgk
-
Purification (Commentary)
Commentary
Organism
native enzyme to apparent homogeneity by using a combination of seven purification steps
Aspergillus niger
Storage Stability
Storage Stability
Organism
-20°C, partly purified enzyme, stable at
Aspergillus niger
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
-
739585
Aspergillus niger
ADP + 6-phospho-alpha-D-glucopyranose
-
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
-
739585
Aspergillus niger
ADP + 6-phospho-alpha-D-glucopyranose
FAB-mass spectrometric method for product identification
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
-
739585
Aspergillus niger CBX-209
ADP + 6-phospho-alpha-D-glucopyranose
-
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
-
739585
Aspergillus niger CBX-209
ADP + 6-phospho-alpha-D-glucopyranose
FAB-mass spectrometric method for product identification
-
-
?
additional information
substrate specificity for levoglucosan, not only the structure of the intramolecular glucosidic linkage but also the configuration of the pyranose frame is specific for recognition by levoglucosan kinase
739585
Aspergillus niger
?
-
-
-
-
additional information
substrate specificity for levoglucosan, not only the structure of the intramolecular glucosidic linkage but also the configuration of the pyranose frame is specific for recognition by levoglucosan kinase
739585
Aspergillus niger CBX-209
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 56000, SDS-PAGE
Aspergillus niger
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Aspergillus niger
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
levoglucosan can protect the enzyme from thermal inactivation
Aspergillus niger
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
-
Aspergillus niger
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
partly purified enzyme, stable at
Aspergillus niger
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Aspergillus niger
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.2
-
ADP
pH 9.3, 30°C
Aspergillus niger
Cloned(Commentary) (protein specific)
Commentary
Organism
gene lgk, recombinant expression in Escherichia coli strain DH5alpha
Aspergillus niger
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Aspergillus niger
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
competitive inhibition
Aspergillus niger
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.2
-
ADP
pH 9.3, 30°C
Aspergillus niger
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
ATP
pH 9.3, 30°C
Aspergillus niger
71.2
-
1,6-anhydro-beta-D-glucopyranose
pH 9.3, 30°C
Aspergillus niger
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Aspergillus niger
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
x * 56000, SDS-PAGE
Aspergillus niger
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
Aspergillus niger
-
ADP + 6-phospho-alpha-D-glucopyranose
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
Aspergillus niger CBX-209
-
ADP + 6-phospho-alpha-D-glucopyranose
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme to apparent homogeneity by using a combination of seven purification steps
Aspergillus niger
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, partly purified enzyme, stable at
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
-
739585
Aspergillus niger
ADP + 6-phospho-alpha-D-glucopyranose
-
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
-
739585
Aspergillus niger
ADP + 6-phospho-alpha-D-glucopyranose
FAB-mass spectrometric method for product identification
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
-
739585
Aspergillus niger CBX-209
ADP + 6-phospho-alpha-D-glucopyranose
-
-
-
?
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O
-
739585
Aspergillus niger CBX-209
ADP + 6-phospho-alpha-D-glucopyranose
FAB-mass spectrometric method for product identification
-
-
?
additional information
substrate specificity for levoglucosan, not only the structure of the intramolecular glucosidic linkage but also the configuration of the pyranose frame is specific for recognition by levoglucosan kinase
739585
Aspergillus niger
?
-
-
-
-
additional information
substrate specificity for levoglucosan, not only the structure of the intramolecular glucosidic linkage but also the configuration of the pyranose frame is specific for recognition by levoglucosan kinase
739585
Aspergillus niger CBX-209
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 56000, SDS-PAGE
Aspergillus niger
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Aspergillus niger
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
levoglucosan can protect the enzyme from thermal inactivation
Aspergillus niger
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
-
Aspergillus niger
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
partly purified enzyme, stable at
Aspergillus niger
Other publictions for EC 2.7.1.B28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738435
Bacik
Bioconversion of anhydrosugars ...
Alternaria alternata, Aspergillus niger, Aspergillus niger CBX-209, Aspergillus terreus, Buckleyzyma aurantiaca, Hannaella luteola, Lipomyces starkeyi, Lipomyces starkeyi YZ-215, Papiliotrema flavescens, Papiliotrema laurentii, Penicillium herquei, Penicillium javanicum, Sporidiobolus salmonicolor
IUBMB Life
68
700-708
2016
-
-
2
-
1
-
4
22
-
12
-
13
-
16
-
-
2
2
-
-
-
1
18
-
2
-
1
-
2
-
2
11
1
-
3
-
-
2
11
-
1
-
3
4
1
22
-
12
-
13
-
-
-
2
-
-
-
1
18
-
2
-
1
-
2
-
2
-
-
1
1
-
-
-
738664
Bacik
Producing glucose 6-phosphate ...
Lipomyces starkeyi, Lipomyces starkeyi YZ-215
J. Biol. Chem.
290
26638-26648
2015
-
-
1
1
-
-
-
-
-
1
-
2
-
3
-
-
1
1
-
-
-
-
6
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
738937
Dai
Computational analysis of AnmK ...
Aspergillus terreus, Lipomyces starkeyi
J. Theor. Biol.
379
59-65
2015
-
-
2
-
-
-
-
-
-
2
-
2
-
7
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
738262
Xie
Screening and identification o ...
Aspergillus niger
FEMS Microbiol. Lett.
251
313-319
2005
-
-
1
-
-
-
-
-
-
1
1
1
-
6
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
739585
Zhuang
Identification, characterizati ...
Aspergillus niger, Aspergillus niger CBX-209
Protein Expr. Purif.
1
71-81
2002
-
-
1
-
-
-
1
2
-
1
1
2
-
8
-
-
1
-
-
-
-
1
6
1
1
-
1
-
1
-
1
1
1
-
-
-
-
1
1
-
-
-
-
1
1
2
-
1
1
2
-
-
-
1
-
-
-
1
6
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
739710
Zhuang
Characterization of inductive ...
Aspergillus niger, Aspergillus niger CBX-209
Sheng Wu Gong Cheng Xue Bao
5
583-587
2002
-
-
-
-
-
-
-
-
-
1
-
2
-
5
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-